Rabbit anti-Mouse, Rat mGluR4 Polyclonal Antibody | anti-GRM4 antibody

mGluR4 (glutamate receptor, metabotropic 4, Gprc1d, Metabotropic glutamate receptor 4, Mglur4)

Cat. Num. AAA619346

• Gene Names: GRM4; mGlu4; GPRC1D; MGLUR4; MGC177594
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: mGluR4; Polyclonal Antibody; mGluR4 (glutamate receptor; metabotropic 4; Gprc1d; Metabotropic glutamate receptor 4; Mglur4); Anti -mGluR4 (glutamate receptor; anti-GRM4 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mGluR4 (GRM4). Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-GRM4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:5000
• Immunogen: Recombinant mGluR4.
• Positive Control: Mouse Brain Lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: (Representative Lot Data) Mouse brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-mGluR4 (1:5,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates mGluR4)

Western Blot (WB)

(Western Blot: Rat Cerebellum lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-mGluR4 (1:5,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates mGluR4 (101kDa))

Related Product Information for anti-GRM4 antibody

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Product Categories/Family for anti-GRM4 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4504141
• NCBI GeneID: 2914
• NCBI Accession #: NP_000832.1
• NCBI GenBank Nucleotide #: NM_000841.1
• UniProt Accession #: Q14833; Q5SZ84; Q59GK5; Q6ZMQ2; AAB34891
• Molecular Weight: 101,868 Da
• NCBI Official Full Name: metabotropic glutamate receptor 4
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 4
• NCBI Official Symbol: GRM4
• NCBI Official Synonym Symbols: mGlu4; GPRC1D; MGLUR4; MGC177594
• NCBI Protein Information: metabotropic glutamate receptor 4; OTTHUMP00000016221; glutamate metabotropic receptor 4
• UniProt Protein Name: Metabotropic glutamate receptor 4
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM4
• UniProt Synonym Gene Names: GPRC1D; MGLUR4
• UniProt Entry Name: GRM4_HUMAN

NCBI Description

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq]

Uniprot Description

mGluR4: metabotropic glutamate receptor 3, a G-protein coupled receptor for glutamate. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Cell development/differentiation; Membrane protein, integral; GPCR, family 3

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: presynaptic membrane; integral to plasma membrane; plasma membrane; terminal button; presynaptic active zone membrane; dendritic shaft; cytoplasmic vesicle; intracellular

Molecular Function: calmodulin binding; G-protein coupled receptor activity; group III metabotropic glutamate receptor activity; glutamate receptor activity; calcium-dependent protein binding

Biological Process: synaptic transmission; regulation of synaptic transmission, glutamatergic; positive regulation of MAPKKK cascade; activation of MAPK activity; neurotransmitter secretion; regulation of neuron apoptosis; learning; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway