Immunoprecipitation (IP)
(Immunoprecipitation: 10ug of this antibody immunoprecipitated a-Insulin Receptor, beta subunit from 500ug of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, b subunit (97kD).)
Rabbit a-Insulin Receptor, beta subunit Polyclonal Antibody
a-Insulin Receptor, beta subunit (INSR, Insulin receptor)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
a-Insulin Receptor; beta subunit; Polyclonal Antibody; beta subunit (INSR; Insulin receptor); Anti -a-Insulin Receptor; anti-a-Insulin Receptor; beta subunit antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human a-Insulin Receptor, beta subunit. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-a-Insulin Receptor, beta subunit antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
GST-fusion protein corresponding to the last 100 C-terminal amino acid residues of the human insulin receptor beta chain.
Positive Control
Human brain tissue lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunoprecipitation (IP)
(Immunoprecipitation: 10ug of this antibody immunoprecipitated a-Insulin Receptor, beta subunit from 500ug of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, b subunit (97kD).)
Immunoprecipitation (IP)
(Immunoprecipitation: 10ug of this antibody immunoprecipitated a-Insulin Receptor, beta subunit from 500ug of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, b subunit (97kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor,_b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor,_b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kD).)
Related Product Information for anti-a-Insulin Receptor, beta subunit antibody
The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular alpha-chain and a transmembrane and intracellular beta-chain, tethered together by disulfide bonds. The beta-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.
Product Categories/Family for anti-a-Insulin Receptor, beta subunit antibody
Similar Products
Product Notes
The a-Insulin Receptor, beta subunit (Catalog #AAA619232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The a-Insulin Receptor, beta subunit (INSR, Insulin receptor) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's a-Insulin Receptor, beta subunit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the a-Insulin Receptor, beta subunit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "a-Insulin Receptor, beta subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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