Western Blot (WB)
(Western blot analysis of GATA3 was performed by loading 15ug of SH-SY5Y whole cell lysate (WCL) and SH-SY5Y nuclear extract (NE) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked for at least 1 hour. The membrane was probed with Mouse anti GATA3 at a dilution of 1/1000 overnight at 4 degree C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody for 1 hour. Chemiluminescent detection was performed)
Mouse GATA3 Monoclonal Antibody | anti-GATA3 antibody
Mouse anti GATA3
IF: 1:50-1:100
IP: 5 ug
WB: 1:1000
IHC-Paraffin: 1:200-1:2000
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of GATA3 was performed by loading 15ug of SH-SY5Y whole cell lysate (WCL) and SH-SY5Y nuclear extract (NE) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked for at least 1 hour. The membrane was probed with Mouse anti GATA3 at a dilution of 1/1000 overnight at 4 degree C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody for 1 hour. Chemiluminescent detection was performed)
Western Blot (WB)
(Western blot analysis of GATA3 was performed by loading 15ug of SH-SY5Y whole cell lysate (WCL) and SH-SY5Y nuclear extract (NE) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked for at least 1 hour. The membrane was probed with Mouse anti GATA3 at a dilution of 1/1000 overnight at 4 degree C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody for 1 hour. Chemiluminescent detection was performed)
Immunofluorescence (IF)
(Immunofluorescent analysis of GATA3 (green) in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) Mouse anti GATA3 at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody for 30 minutes at room temperature. F-Actin (red) was stained with phalloidin (red) and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20x magnification)
Immunofluorescence (IF)
(Immunofluorescent analysis of GATA3 (green) in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) Mouse anti GATA3 at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody for 30 minutes at room temperature. F-Actin (red) was stained with phalloidin (red) and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20x magnification)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA3 showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with dH2O and PBS, and then probed with a Mouse anti GATA3 diluted in 3% BSA-PBS at a dilution of 1/1000 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA3 showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with dH2O and PBS, and then probed with a Mouse anti GATA3 diluted in 3% BSA-PBS at a dilution of 1/1000 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
The GATA3 protein is a member of the GATA family of transcription factors which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (P15976). GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Ho et al. 1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chou et al. 2010).
Aberrant GATA3 levels have also been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Krings et al. 2014).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Uniprot Description
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Research Articles on GATA3
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Product Notes
The GATA3 gata3 (Catalog #AAA225375) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti GATA3 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GATA3 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:50-1:100 IF: 1:50-1:100 IP: 5 ug WB: 1:1000 IHC-Paraffin: 1:200-1:2000. Researchers should empirically determine the suitability of the GATA3 gata3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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