Human Trans-acting T-cell-specific transcription factor GATA-3 ELISA Kit | GATA3 elisa kit

Human Trans-acting T-cell-specific transcription factor GATA-3 ELISA Kit

Cat. Num. AAA2883439

• Gene Names: GATA3; HDR; HDRS
• Reactivity: Human
• Synonyms: Trans-acting T-cell-specific transcription factor GATA-3; Human Trans-acting T-cell-specific transcription factor GATA-3 ELISA Kit; GATA-binding factor 3; GATA3; GATA3 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Trans-acting T-cell-specific transcription factor GATA-3. No significant cross-reactivity or interference was observed.
• Sequence Length: 444

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.31 ng/mL -20.0 ng/mL
• Sensitivity: <0.182 ng/mL.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Trans-acting T-cell-specific transcription factor GATA-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trans-acting T-cell-specific transcription factor GATA-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Trans-acting T-cell-specific transcription factor GATA-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Trans-acting T-cell-specific transcription factor GATA-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Trans-acting T-cell-specific transcription factor GATA-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50541959
• NCBI GeneID: 2625
• NCBI Accession #: NP_001002295.1
• NCBI GenBank Nucleotide #: NM_001002295.1
• UniProt Accession #: P23771; Q5VWG7; Q5VWG8; Q96J16
• Molecular Weight: 48,045 Da
• NCBI Official Full Name: trans-acting T-cell-specific transcription factor GATA-3 isoform 1
• NCBI Official Synonym Full Names: GATA binding protein 3
• NCBI Official Symbol: GATA3
• NCBI Official Synonym Symbols: HDR; HDRS
• NCBI Protein Information: trans-acting T-cell-specific transcription factor GATA-3
• UniProt Protein Name: Trans-acting T-cell-specific transcription factor GATA-3
• Protein Family: Transcription factor
• UniProt Gene Name: GATA3
• UniProt Entry Name: GATA3_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Uniprot Description

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.