Western Blot (WB)
(Figure 1. Western blot analysis using GATA3 mouse mAb against truncated GATA3-His recombinant protein (1).)
Mouse anti-Human GATA3 Antibody | anti-GATA3 antibody
GATA3 Antibody
Gene Names
GATA3; HDR; HDRS
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
GATA3; GATA3 Antibody; HDR; anti-GATA3 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
1A10D1
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
444
Applicable Applications for anti-GATA3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:10000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of GATA3 (aa175-388) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using GATA3 mouse mAb against truncated GATA3-His recombinant protein (1).)
Western Blot (WB)
(Figure 1. Western blot analysis using GATA3 mouse mAb against truncated GATA3-His recombinant protein (1).)
Related Product Information for anti-GATA3 antibody
GATA3. GATA binding protein 3. The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells, macrophages, or HeLa cell lines. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Product Categories/Family for anti-GATA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,045 Da
NCBI Official Full Name
trans-acting T-cell-specific transcription factor GATA-3 isoform 1
NCBI Official Synonym Full Names
GATA binding protein 3
NCBI Official Symbol
GATA3
NCBI Official Synonym Symbols
HDR; HDRS
NCBI Protein Information
trans-acting T-cell-specific transcription factor GATA-3
UniProt Protein Name
Trans-acting T-cell-specific transcription factor GATA-3
Protein Family
UniProt Gene Name
GATA3
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Uniprot Description
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Research Articles on GATA3
Similar Products
Product Notes
The GATA3 gata3 (Catalog #AAA850144) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GATA3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the GATA3 gata3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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