Western Blot (WB)
(Western blot analysis of extracts from A549 cells, untreated or treated with EGF (10ng/ml, 10min), using p44/42 MAP Kinase (Phospho-Thr202) Rabbit pAb (310064, 1:500 diluted, upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246, 1:1000 diluted, lower).)
Rabbit p44/42 MAP Kinase Polyclonal Antibody | anti-p44/42 MAP Kinase antibody
Anti-p44/42 MAP Kinase (Phospho-Thr202) Rabbit pAb
ICC: 1:100-1:200
IHC: 1:50-1:100
Western Blot (WB)
(Western blot analysis of extracts from A549 cells, untreated or treated with EGF (10ng/ml, 10min), using p44/42 MAP Kinase (Phospho-Thr202) Rabbit pAb (310064, 1:500 diluted, upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246, 1:1000 diluted, lower).)
Western Blot (WB)
(Western blot analysis of extracts from A549 cells, untreated or treated with EGF (10ng/ml, 10min), using p44/42 MAP Kinase (Phospho-Thr202) Rabbit pAb (310064, 1:500 diluted, upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246, 1:1000 diluted, lower).)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202) Antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase (Phospho-Thr202) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase (Phospho-Thr202) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202) Antibody (left) or the same antibody preincubated with blocking peptide #51245 (right).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202) Antibody (left) or the same antibody preincubated with blocking peptide #51245 (right).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.
Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: caveola; cytoskeleton; cytosol; early endosome; focal adhesion; Golgi apparatus; late endosome; microtubule cytoskeleton; mitochondrion; nuclear envelope; nucleoplasm; nucleus
Molecular Function: kinase activity; MAP kinase activity; phosphatase binding; protein binding; protein serine/threonine kinase activity
Biological Process: activation of MAPK activity; axon guidance; BMP signaling pathway; DNA damage induced protein phosphorylation; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphorylation; platelet activation; positive regulation of cyclase activity; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; transcription initiation from RNA polymerase I promoter
Research Articles on p44/42 MAP Kinase
Similar Products
Product Notes
The p44/42 MAP Kinase mapk3 (Catalog #AAA477027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p44/42 MAP Kinase (Phospho-Thr202) Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p44/42 MAP Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:500-1:1000 ICC: 1:100-1:200 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the p44/42 MAP Kinase mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p44/42 MAP Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
