Immunofluorescence (IF)
(Immunofluorescent analysis of GATA1 (green) in A431 cells (right) using Rabbit anti GATA1 antibody compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x)
Rabbit anti-Human, Mouse GATA1 Polyclonal Antibody | anti-GATA1 antibody
Rabbit anti GATA1
IF: 1/50-1/500
WB: 1:500
IHC-Paraffin: 1:50-1:500
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Immunofluorescence (IF)
(Immunofluorescent analysis of GATA1 (green) in A431 cells (right) using Rabbit anti GATA1 antibody compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x)
Immunofluorescence (IF)
(Immunofluorescent analysis of GATA1 (green) in A431 cells (right) using Rabbit anti GATA1 antibody compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x)
Immunofluorescence (IF)
(Immunofluorescent analysis of GATA1 (green) in U251 cells (right) using Rabbit anti GATA1 antibody compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x)
Immunofluorescence (IF)
(Immunofluorescent analysis of GATA1 (green) in U251 cells (right) using Rabbit anti GATA1 antibody compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GATA1 polyclonal antibody in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated cells were counterstained with phalloidin (F-actin, red) and Hoechst 33342 (Nucleus, blue). Images were taken at a magnification of 60x)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA1 using Rabbit anti GATA1 antibody showing staining in the nucleus of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA1 using Rabbit anti GATA1 antibody showing staining in the nucleus of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with GATA1 polyclonal antibody diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting)
Western Blot (WB)
(Western blot analysis of GATA1 was performed by loading 25 ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with rabbit polyclonal antibody recognizing GATA1 at a dilution of 1/500 overnight at 4 degree C on a rocking platform. Membranes were then washed in TBS-0.1% Tween 20 and probed with goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and detection was carried out using chemiluminescence)
Western Blot (WB)
(Western blot analysis of GATA1 was performed by loading 25 ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with rabbit polyclonal antibody recognizing GATA1 at a dilution of 1/500 overnight at 4 degree C on a rocking platform. Membranes were then washed in TBS-0.1% Tween 20 and probed with goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and detection was carried out using chemiluminescence)
GATA1 acts as an erythroid transcription factor by regulating erythroid-specific genes. In addition to that, GATA1 has also been implied to play a role in regulating differentiation of other hematopoietic lineages (including mast cells and megakaryocytes) and endothelial cells (Orkin 1992).
GATA1 alterations have been implicated in a variety of diseases, including acute megakaryoblastic leukemia (AMKL) associated with Trisomy 21 and thrombocytopenia (Ciovacco et al. 2008).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).
Research Articles on GATA1
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Product Notes
The GATA1 gata1 (Catalog #AAA225048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti GATA1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GATA1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:50-1:500 IF: 1/50-1/500 WB: 1:500 IHC-Paraffin: 1:50-1:500. Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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