Rabbit GATA3 Polyclonal Antibody | anti-GATA3 antibody

GATA3 Antibody

Cat. Num. AAA150421

• Gene Names: GATA3; HDR; HDRS
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: GATA3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: GATA3; Polyclonal Antibody; GATA3 Antibody; HDR; HDRS; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; GATA binding protein 3; anti-GATA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: GATA3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 444

• Applicable Applications for anti-GATA3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: GATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3.
• Buffer: GATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: GATA3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of GATA3 in K562 cell lysate with GATA3 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of GATA3 in Rat Brain tissue with GATA3 antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of GATA3 in mouse brain tissue with GATA3 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of GATA3 in mouse brain tissue with GATA3 Antibody at 20 μg/mL.)

Related Product Information for anti-GATA3 antibody

GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 50541959
• NCBI GeneID: 2625
• NCBI Accession #: NP_001002295
• NCBI GenBank Nucleotide #: NM_001002295.1
• UniProt Accession #: P23771; Q5VWG7; Q5VWG8; Q96J16
• Molecular Weight: 48,045 Da
• NCBI Official Full Name: trans-acting T-cell-specific transcription factor GATA-3 isoform 1
• NCBI Official Synonym Full Names: GATA binding protein 3
• NCBI Official Symbol: GATA3
• NCBI Official Synonym Symbols: HDR; HDRS
• NCBI Protein Information: trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3
• UniProt Protein Name: Trans-acting T-cell-specific transcription factor GATA-3
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA3
• UniProt Entry Name: GATA3_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Uniprot Description

GATA3: a transcriptional regulator central in Th2 cell differentiation. Inhibits breast cancer growth and pulmonary breast cancer metastasis. Represses INK4C transcription, constrains luminal progenitor cell expansion, and suppresses luminal tumorigenesis in the mammary gland. High GATA3 and low INK4C expression predicts a favorable patient outcome in luminal A type breast tumors. IFN-lambda1 (IL-29) inhibits GATA3 expression and suppresses Th2 responses in human T cells. GATA3 haploinsufficiency leads to HDR (hypoparathyroidism, deafness, and renal dysplasia) syndrome. Two alternatively spliced human isoforms have been described.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; zinc ion binding; interleukin-2 receptor binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: developmental growth; positive regulation of transcription, DNA-dependent; cell maturation; ear development; cell fate determination; uterus development; T cell receptor signaling pathway; negative regulation of interleukin-2 production; post-embryonic development; norepinephrine biosynthetic process; regulation of neuron apoptosis; anatomical structure formation; erythrocyte differentiation; mesonephros development; kidney development; regulation of cytokine biosynthetic process; response to drug; thymic T cell selection; inner ear morphogenesis; anatomical structure morphogenesis; positive regulation of signal transduction; embryonic hemopoiesis; response to virus; negative regulation of fat cell differentiation; parathyroid gland development; negative regulation of mammary gland epithelial cell proliferation; response to ethanol; positive regulation of T cell differentiation; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; pro-T cell differentiation; negative regulation of transcription, DNA-dependent; regulation of CD4-positive, alpha beta T cell differentiation; transcription from RNA polymerase II promoter; lens development in camera-type eye; axon guidance; phosphoinositide 3-kinase cascade; TOR signaling pathway; neuron migration; defense response; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of cell cycle; positive regulation of interleukin-4 production; negative regulation of cell proliferation; sympathetic nervous system development; negative regulation of interferon-gamma production; response to gamma radiation; type IV hypersensitivity; pharyngeal system development; thymus development; in utero embryonic development; male gonad development; humoral immune response; positive regulation of protein kinase B signaling cascade; negative regulation of inflammatory response; innate immune response; regulation of histone H3-K4 methylation; T-helper 2 cell differentiation; blood coagulation

Disease: Hypoparathyroidism, Sensorineural Deafness, And Renal Disease