Flow Cytometry (FC/FACS)
(Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.5 ug CD266 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were analyzed.)
Mouse Fn14 Monoclonal Antibody | anti-Fn14 antibody
Fn14 antibody
Gene Names
tnfrsf12a; fn14
Applications
Functional Assay
Purity
Fn14 antibody was purified by affinity chromatography.
Synonyms
Fn14; Monoclonal Antibody; Fn14 antibody; Monoclonal Fn14; Anti-Fn14; TNFRSF12A; Fn-14; TWEAKR; Tumor necrosis factor ligand superfamily member 12; Fn 14; Fibroblast growth factor-inducible 14; anti-Fn14 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
ITEM-4
Specificity
Mouse, Human
Purity/Purification
Fn14 antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Sequence Length
120
Applicable Applications for anti-Fn14 antibody
Functional Assay
Application Notes
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/ml. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg.
Biological Significance
The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14. Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.5 ug CD266 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were analyzed.)
Flow Cytometry (FC/FACS)
(Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.5 ug CD266 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were analyzed.)
Related Product Information for anti-Fn14 antibody
Mouse monoclonal Fn14 antibody
Product Categories/Family for anti-Fn14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,295 Da
NCBI Official Full Name
Fn14
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 12A<
NCBI Official Symbol
tnfrsf12a
NCBI Official Synonym Symbols
fn14
NCBI Protein Information
tumor necrosis factor receptor superfamily, member 12A
UniProt Protein Name
Fn14
UniProt Gene Name
tnfrsf12a
UniProt Entry Name
Q6SIX7_XENLA
Research Articles on Fn14
Similar Products
Product Notes
The Fn14 tnfrsf12a (Catalog #AAA832619) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fn14 can be used in a range of immunoassay formats including, but not limited to, Functional Assay. The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/ml. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Researchers should empirically determine the suitability of the Fn14 tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fn14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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