Western Blot (WB)
(Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 μg/mL.)
Rabbit anti-Human Fn14 Polyclonal Antibody | anti-TNFRSF12A antibody
Fn14 Antibody
Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Fn14 Antibody is affinity chromatography purified via peptide column.
Synonyms
Fn14; Polyclonal Antibody; Fn14 Antibody; FN14; CD266; TWEAKR; Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; tumor necrosis factor receptor superfamily; member 12A; anti-TNFRSF12A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Fn14 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
129
Applicable Applications for anti-TNFRSF12A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Fn14 antibody can be used for the detection of Fn14 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
Fn14 antibody was raised against a human IgG1 Fc-tagged Fn14 protein.
Buffer
Fn14 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Fn14 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 μg/mL.)
Western Blot (WB)
(Western blot analysis of Fn14 in HepG2 cells with Fn14 antibody at (A) 2 and (B) 4 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of Fn14 in human liver tissue with Fn14 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Fn14 in Human Liver cells with Fn14 antibody at 20 μg/mL.)
Related Product Information for anti-TNFRSF12A antibody
Fn14 Antibody: TWEAK is a TNF family member produced by IFN-gamma-stimulated monocytes and induces multiple cell death pathways including caspase-dependent apoptosis and cathepsin B-dependent necrosis. These pathways are mediated by the binding of TWEAK by fibroblast growth factor 14 (Fn14), a member of the TNF receptor superfamily. Yeast two-hybrid experiments have shown that FN14 will bind to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Fn14 is induced during liver regeneration and is highly expressed in hepatocellular carcinomas. In addition, TWEAK induces liver progenitor cell proliferation, suggesting Fn14 may play a role in hepatocyte growth control and liver neoplasia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,191 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 12A
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 12A
NCBI Official Symbol
TNFRSF12A
NCBI Official Synonym Symbols
FN14; CD266; TWEAKR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 12A; tweak-receptor; FGF-inducible 14; type I transmembrane protein Fn14; fibroblast growth factor-inducible immediate-early response protein 14
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 12A
Protein Family
UniProt Gene Name
TNFRSF12A
UniProt Synonym Gene Names
FN14; FGF-inducible 14; TweakR
UniProt Entry Name
TNR12_HUMAN
Uniprot Description
TweakR: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. Associates with TRAF1 and TRAF2, and probably also with TRAF3. By FGF1 and phorbol ester. Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell development/differentiation; Receptor, misc.
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: ruffle; cell surface; integral to membrane; plasma membrane
Molecular Function: protein binding
Biological Process: positive regulation of apoptosis; substrate-bound cell migration, cell attachment to substrate; positive regulation of axon extension; angiogenesis; regulation of angiogenesis
Research Articles on TNFRSF12A
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Product Notes
The TNFRSF12A tnfrsf12a (Catalog #AAA150342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fn14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fn14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Fn14 antibody can be used for the detection of Fn14 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the TNFRSF12A tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fn14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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