Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) Standard Curve.)
Human Tumor Necrosis Factor Receptor Superfamily Member 12A (TNFRSF12A) ELISA Kit | TNFRSF12A elisa kit
Human Tumor Necrosis Factor Receptor Superfamily Member 12A (TNFRSF12A) ELISA Kit
Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Reactivity
Human
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 12A (TNFRSF12A); Human Tumor Necrosis Factor Receptor Superfamily Member 12A (TNFRSF12A) ELISA Kit; TNFRSF12A; CD266; FN14; TWEAKR; type I transmembrane protein Fn14; TNFRSF12A elisa kit
Reactivity
Human
Specificity
Human Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF12A. No significant cross-reactivity or interference between Human TNFRSF12A and analogues was observed.
Sequence Length
129
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) Standard Curve.)
Related Product Information for TNFRSF12A elisa kit
Description: This Human Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF12A in samples. An antibody specific for TNFRSF12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFRSF12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF12A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF12A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: FN14 protein, which shares 82% amino acid identity with the mouse sequence, contains a signal peptide, an extracellular domain, a membrane-anchoring domain, and a cytoplasmic domain. Northern blot analysis detected increased FN14 expression in response to FGF1, calf serum, or phorbol ester stimulation of human quiescent fibroblasts in vitro. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated FN14 expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. Expression of mouse Fn14 was upregulated in hepatocellular carcinoma nodules that develop in 2 different transgenic mouse models of hepatocarcinogenesis.
Background: FN14 protein, which shares 82% amino acid identity with the mouse sequence, contains a signal peptide, an extracellular domain, a membrane-anchoring domain, and a cytoplasmic domain. Northern blot analysis detected increased FN14 expression in response to FGF1, calf serum, or phorbol ester stimulation of human quiescent fibroblasts in vitro. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated FN14 expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. Expression of mouse Fn14 was upregulated in hepatocellular carcinoma nodules that develop in 2 different transgenic mouse models of hepatocarcinogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 12A
NCBI Official Synonym Full Names
TNF receptor superfamily member 12A
NCBI Official Symbol
TNFRSF12A
NCBI Official Synonym Symbols
FN14; CD266; TWEAKR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 12A
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 12A
Protein Family
UniProt Gene Name
TNFRSF12A
UniProt Synonym Gene Names
FN14; FGF-inducible 14; TweakR
UniProt Entry Name
TNR12_HUMAN
Uniprot Description
TweakR: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. Associates with TRAF1 and TRAF2, and probably also with TRAF3. By FGF1 and phorbol ester. Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell development/differentiation
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: ruffle; cell surface; plasma membrane; integral to membrane
Molecular Function: protein binding
Biological Process: substrate-bound cell migration, cell attachment to substrate; positive regulation of apoptosis; positive regulation of axon extension; angiogenesis; regulation of angiogenesis
Research Articles on TNFRSF12A
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Product Notes
The Human TNFRSF12A tnfrsf12a (Catalog #AAA9713890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713890 ELISA Kit recognizes Human TNFRSF12A. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily Member 12A (TNFRSF12A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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