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Testing Data #1 (Human CD266 transfected cells stained with Mouse anti Human CD266 (MBS224691) followed by Rabbit F(ab')2 anti Mouse IgG:RPE (MBS235280))

Mouse CD266 Monoclonal Antibody | anti-CD266 antibody

MOUSE ANTI HUMAN CD266:FITC

Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Applications
Flow Cytometry, Functional Assay
Synonyms
CD266; Monoclonal Antibody; MOUSE ANTI HUMAN CD266:FITC; anti-CD266 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
ITEM-4
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Sequence Length
94
Applicable Applications for anti-CD266 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation
Immunogen
Human CD266 transfected P815 cells
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human CD266 transfected cells stained with Mouse anti Human CD266 (MBS224691) followed by Rabbit F(ab')2 anti Mouse IgG:RPE (MBS235280))

Testing Data #1 (Human CD266 transfected cells stained with Mouse anti Human CD266 (MBS224691) followed by Rabbit F(ab')2 anti Mouse IgG:RPE (MBS235280))

Testing Data #2

(Human CD266 transfected cells stained with Mouse anti Human CD266:FITC (MBS224675) )

Testing Data #2 (Human CD266 transfected cells stained with Mouse anti Human CD266:FITC (MBS224675) )

Testing Data #3

(Human CD266 transfected cells stained with Mouse anti Human CD266: Alexa FluorR488 (MBS224691))

Testing Data #3 (Human CD266 transfected cells stained with Mouse anti Human CD266: Alexa FluorR488 (MBS224691))

Testing Data #4

(Human CD266 transfected cells stained with Mouse anti Human CD266: Alexa FluorR647 (MBS224691))

Testing Data #4 (Human CD266 transfected cells stained with Mouse anti Human CD266: Alexa FluorR647 (MBS224691))

Testing Data #5

(Human CD266 transfected cells stained with Mouse anti Human CD266:RPE (MBS224572))

Testing Data #5 (Human CD266 transfected cells stained with Mouse anti Human CD266:RPE (MBS224572))
Related Product Information for anti-CD266 antibody
Mouse anti Human CD266 antibody, clone ITEM-4 recognizes human CD266, otherwise known as Fn14/TWEAK Receptor, a 14kDa type I transmembrane glycoprotein and member of the tumour necrosis factor receptor superfamily (TNFRSF12A) expressed by a variety of tissues, including the heart, lung and kidney, and at elevated levels by human brain and liver tumours. The interaction between CD266 and its ligand CD255 (TWEAK), induces the proliferation and migration of endothelial cells and also angiogenesis, and studies have shown that CD255-induced apoptosis of some tumour cell lines is mediated by CD266 (Nakayama et al. 2003). Mouse anti Human CD266 antibody, clone ITEM-4 is cross-reactive with mouse CD266, and also with Rat-1 cells in flow cytometry.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,191 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 12A
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 12A
NCBI Official Symbol
TNFRSF12A
NCBI Official Synonym Symbols
FN14; CD266; TWEAKR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 12A; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; tweak-receptor; type I transmembrane protein Fn14
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 12A
UniProt Gene Name
TNFRSF12A
UniProt Synonym Gene Names
FN14; FGF-inducible 14; TweakR
UniProt Entry Name
TNR12_HUMAN

Uniprot Description

TweakR: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. Associates with TRAF1 and TRAF2, and probably also with TRAF3. By FGF1 and phorbol ester. Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell development/differentiation; Receptor, misc.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: ruffle; cell surface; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; substrate-bound cell migration, cell attachment to substrate; positive regulation of axon extension; angiogenesis; regulation of angiogenesis

Research Articles on CD266

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Product Notes

The CD266 tnfrsf12a (Catalog #AAA224369) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD266 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD266 tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD266, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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