TNFRSF12A / FN14 / TWEAKR recombinant protein

Recombinant Human TNFRSF12A / FN14 / TWEAKR Protein (Fc tag)

Cat. Num. AAA2546263

• Gene Names: TNFRSF12A; FN14; CD266; TWEAKR
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TNFRSF12A / FN14 / TWEAKR; Recombinant Human TNFRSF12A / FN14 / TWEAKR Protein (Fc tag); CD266; FN14; TWEAKR; TNFRSF12A / FN14 / TWEAKR recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 129

• Application Notes: The recombinant human TNFRSF12A/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 313 amino acids and has a calculated molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTNFRSF12A/Fc monomer is approximately 37 kDa.
• Predicted N Terminal: Glu
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TNFRSF12A / FN14 / TWEAKR recombinant protein

Background: Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Fn14 shares 82% amino acid identity with the mouse sequence. It contains a signal peptide, an extracellular domain, a membrane-anchoring domain, and a cytoplasmic domain. In response to FGF1, calf serum, or phorbol ester stimulation of human quiescent fibroblasts in vitro, the level of Fn14 is increased. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated FN14 expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. Expression of mouse Fn14 was upregulated in hepatocellular carcinoma nodules that develop in 2 different transgenic mouse models of hepatocarcinogenesis. TNFRSF12A is the weak inducer of apoptosis in some cell type s. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.

Description: A DNA sequence encoding the human TNFRSF12A isoform 1 (Q9NP84-1) extracellular domain (Glu 28-Trp 79) was fused with the Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7706186
• NCBI GeneID: 51330
• NCBI Accession #: NP_057723.1
• NCBI GenBank Nucleotide #: NM_016639.2
• UniProt Accession #: Q9NP84; Q9HCS0; D3DUA6
• Molecular Weight: 10,191 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 12A
• NCBI Official Synonym Full Names: TNF receptor superfamily member 12A
• NCBI Official Symbol: TNFRSF12A
• NCBI Official Synonym Symbols: FN14; CD266; TWEAKR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 12A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 12A
• UniProt Gene Name: TNFRSF12A
• UniProt Synonym Gene Names: FN14; FGF-inducible 14; TweakR

Uniprot Description

TweakR: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. Associates with TRAF1 and TRAF2, and probably also with TRAF3. By FGF1 and phorbol ester. Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; tumor necrosis factor-mediated signaling pathway