anti-Mouse FceR1 alpha Monoclonal Antibody | anti-FceR1alpha antibody
Anti-FceR1 alpha [Mar-1], Rabbit IgG, Lambda
Gene Names
Fcer1a; FcERI; Fce1a; Fcr-5; fcepsilonri
Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
FceR1 alpha; Monoclonal Antibody; Anti-FceR1 alpha [Mar-1]; Rabbit IgG; Lambda; Fcer1a; Fc epsilon RI alpha; FceRIa; FceRI alpha; FceRI-alpha; high affinity IgE receptor; High affinity immunoglobulin epsilon receptor subunit alpha;Fce1a; Fc-epsilon RI-alpha; FcERI; IgE Fc receptor subunit alpha; anti-FceR1alpha antibody
Host
Hamster (Armenian)
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, Lambda
Clone Number
Mar-1
Specificity
The antibody specifically targets the non-signalling alpha chain of the FceR1 complex.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-FceR1alpha antibody
Deplete, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004; Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).
Antibody Type
Recombinant Antibody
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Armenian Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for anti-FceR1alpha antibody
Describes the original use of this antibody in immunohistochemical analysis of epidermal sheets.
Recombinant monoclonal antibody to FceR1 alpha. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mar-1.
Recombinant monoclonal antibody to FceR1 alpha. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mar-1.
References
Holzmann et al.; J Invest Dermatol. 2004 May;122(5):1165-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,657 Da
NCBI Official Full Name
high affinity immunoglobulin epsilon receptor subunit alpha
NCBI Official Synonym Full Names
Fc receptor, IgE, high affinity I, alpha polypeptide
NCBI Official Symbol
Fcer1a
NCBI Official Synonym Symbols
FcERI; Fce1a; Fcr-5; fcepsilonri
NCBI Protein Information
high affinity immunoglobulin epsilon receptor subunit alpha
UniProt Protein Name
High affinity immunoglobulin epsilon receptor subunit alpha
UniProt Gene Name
Fcer1a
UniProt Synonym Gene Names
Fce1a; FcERI
Uniprot Description
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Research Articles on FceR1alpha
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Product Notes
The FceR1alpha fcer1a (Catalog #AAA488523) is an Antibody produced from Hamster (Armenian) and is intended for research purposes only. The product is available for immediate purchase. The Anti-FceR1 alpha [Mar-1], Rabbit IgG, Lambda reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FceR1 alpha can be used in a range of immunoassay formats including, but not limited to, Deplete, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004; Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014). Researchers should empirically determine the suitability of the FceR1alpha fcer1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FceR1 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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