MST1 recombinant protein

Recombinant MST1 protein

Cat. Num. AAA388083

• Gene Names: STK4; KRS2; MST1; YSK3; DKFZp686A2068
• Synonyms: MST1; Recombinant MST1 protein; Mammalian STE20-Like Protein Kinase 1; STK4; KRS2; YSK3; TIIAC; MST1 recombinant protein

• Host: Baculovirus
• Form/Format: 10ug recombinant Mst1 expressed in Sf9 cells and supplied in a buffer of 45mM Tris-HCl, pH8.0, 124mM NaCl, 2.4mM KCl, 3mM DTT, 18mM glutathione, and 10% glycerol.

• Protein Species: Human
• Tag: GST-Tag
• Protein Details: Amino acids 2-488 of MST1 (accession number NP_006273.1) were expressed with an N-terminal GST-Tag, (82kDa) in Sf9 cells using a baculovirus expression system.
• Notes: Recombinant MST1 is suitable for use in kinase assays. A good starting point is 5 to 25ng of enzyme per assay.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(MST1 protein gel. MST1 (6 ?g) run on an SDS-PAGE gel and stained with Coomassie blue.)

Testing Data

(MST1 activity assay. Recombinant MST1 activity measured using a fluorescent kinase assay.)

Related Product Information for MST1 recombinant protein

Short Description: MST1 (Mammalian STE20-like protein kinase 1, STK4, KRS2) is a kinase that phosphorylates histone H2B at serine 14 during apoptosis and in response to double-strand DNA breakage. Amino acids 2-488 of MST1 (accession number NP_006273.1) were expressed with an N-terminal GST-Tag, (MW = 82 kDa) in Sf9 cells using a baculovirus expression system. Recombinant MST1 protein can be used in kinase assays.

Background: MST1 (Mammalian STE20-Like Protein Kinase 1) is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates Ser-14 of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on Ser-212 and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on Ser-650 and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

Product Categories/Family for MST1 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 5454096
• NCBI GeneID: 6789
• NCBI Accession #: NP_006273.1
• NCBI GenBank Nucleotide #: NM_006282.2
• UniProt Accession #: Q13043; Q15802; Q4G156; Q5H982; Q6PD60; Q9BR32; Q9NTZ4; B2RCR8; Q15452; Q59FZ4; Q5H9T5; Q9BS84
• Molecular Weight: 55,630 Da
• NCBI Official Full Name: serine/threonine-protein kinase 4
• NCBI Official Synonym Full Names: serine/threonine kinase 4
• NCBI Official Symbol: STK4
• NCBI Official Synonym Symbols: KRS2; MST1; YSK3; DKFZp686A2068
• NCBI Protein Information: serine/threonine-protein kinase 4; MST-1; OTTHUMP00000043418; OTTHUMP00000063226; STE20-like kinase MST1; mammalian sterile 20-like 1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; serine/threonine-protein kinase Krs-2; dJ211D12.2
• UniProt Protein Name: Serine/threonine-protein kinase 4
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: STK4
• UniProt Synonym Gene Names: KRS2; MST1; MST-1; MST1/N; MST1/C

Uniprot Description

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.