LTBR recombinant protein

LTBR Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123370

• Purity: >85% as determined by SDS-PAGE
• Synonyms: LTBR; LTBR Protein; Rat; Recombinant (hFc Tag); Rat LTBR/TNFRSF3 Protein (Fc Tag); lymphotoxin beta receptor (TNFR superfamily; member 3); LTBR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala218

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 28
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat LTBR (Q5U2S8)(Met1-Ala218) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LTBR recombinant protein

Background: LTBR (lymphotoxin beta receptor (TNFR superfamily, member 3)) is a member of the tumor necrosis factor (TNF) family of receptors. The tumor necrosis factor receptor is a trimeric cytokine receptor that binds tumor necrosis factors. The receptor cooperates with an adaptor protein (such as TRADD, TRAF, RIP), which is important in determining the outcome of the response. LTBR is expressed on the surface of most cell types, including cells of epithelial and myeloid lineages, but not on T and B lymphocytes. LTBR specifically binds the lymphotoxin membrane form (a complex of lymphotoxin-alpha and lymphotoxin-beta). LTBR and its ligand play a role in the development and organization of lymphoid tissue and transformed cells. Activation of this protein can trigger apoptosis. Not only does the LTBR help trigger apoptosis, but it can also lead to the release of the cytokine interleukin 8. Overexpression of LTBR in HEK293 cells increases IL-8 promoter activity and leads to IL-8 release. It is also essential for the development and organization of the secondary lymphoid organs and chemokine release.

References

Summers deLuca L, et al. (2011) A LTbetaR signaling in dendritic cells induces a type I IFN response that is required for optimal clonal expansion of CD8+ T cells. Proc Natl Acad Sci. 108(5):2046-51.Bista P, et al. (2010) TRAF3 controls activation of the canonical and alternative NFkappaB by the lymphotoxin beta receptor. J Biol Chem. 285(17):12971-8.Xu Y, et al. (2011) Adiponectin inhibits lymphotoxin-beta receptor-mediated NF-kappaB signaling in human umbilical vein endothelial cells. Biochem Biophys Res Commun. 404(4):1060-4.

NCBI and Uniprot Product Information

• NCBI GI #: 56605710
• NCBI GeneID: 297604
• NCBI Accession #: NP_001008316.1
• NCBI GenBank Nucleotide #: NM_001008315.1
• UniProt Accession #: Q5U2S8
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 3
• NCBI Official Synonym Full Names: lymphotoxin beta receptor
• NCBI Official Symbol: Ltbr
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 3
• UniProt Protein Name: Lymphotoxin B receptor
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Ltbr