Western Blot (WB)
(Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).)
Rat EB3 Monoclonal Antibody | anti-EB3 antibody
EB3 (KT53) Antibody
Gene Names
Mapre3; EB2; EB3; AI790651
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Protein G chromatography
Synonyms
EB3; Monoclonal Antibody; EB3 (KT53) Antibody; EB3 1; anti-EB3 antibody
Host
Rat
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KT53
Specificity
The antibody detects a 32kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.
Purity/Purification
Protein G chromatography
Form/Format
Rat monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline and 0.05% sodium azide.
Applicable Applications for anti-EB3 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:100
WB: 1:1000
ICC: 1:100
WB: 1:1000
Immunogen
Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3.
Preparation and Storage
Aliquot and store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).)
Western Blot (WB)
(Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).)
Related Product Information for anti-EB3 antibody
Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. These activities of EB proteins are important for mitotic spindle formatin and chromsome alighnment during mitosis, and may have important roles in the centrosome and cilia assembly.
References
Komarova, Y. et al. (2012) Mol Cell. 48:914.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,966 Da
NCBI Official Full Name
microtubule-associated protein RP/EB family member 3
NCBI Official Synonym Full Names
microtubule-associated protein, RP/EB family, member 3
NCBI Official Symbol
Mapre3
NCBI Official Synonym Symbols
EB2; EB3; AI790651
NCBI Protein Information
microtubule-associated protein RP/EB family member 3; RP3; EBF3; end-binding protein 3; EB1 protein family member 3
UniProt Protein Name
Microtubule-associated protein RP/EB family member 3
UniProt Gene Name
Mapre3
UniProt Synonym Gene Names
EBF3; EB3
UniProt Entry Name
MARE3_MOUSE
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Product Notes
The EB3 mapre3 (Catalog #AAA474334) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The EB3 (KT53) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the EB3 mapre3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EB3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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