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Immunoprecipitation (IP) (DHRS2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate4 uL anti-DHRS2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DHRS2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 26 kDa)

Rabbit anti-Human DHRS2 Polyclonal Antibody | anti-DHRS2 antibody

Anti-DHRS2 Antibody, Rabbit Polyclonal

Gene Names
DHRS2; HEP27; SDR25C1
Reactivity
Human
Applications
Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
DHRS2; Polyclonal Antibody; Anti-DHRS2 Antibody; Rabbit Polyclonal; DHRS2 Antibody; Rabbit PAb; Antigen Affinity Purified; dehydrogenase/reductase (SDR family) member 2; Anti-HEP27 Antibody; Anti-SDR25C1 Antibody; anti-DHRS2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DHRS2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DHRS2 antibody
Immunoprecipitation (IP)
Application Notes
IP: 1-4uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human DHRS2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human DHRS2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(DHRS2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate4 uL anti-DHRS2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DHRS2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 26 kDa)

Immunoprecipitation (IP) (DHRS2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate4 uL anti-DHRS2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DHRS2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 26 kDa)
Related Product Information for anti-DHRS2 antibody
Esophageal squamous cell carcinoma (ESCC) is highly prevailing in Asia and it is ranked in the most aggressive squamous cell carcinomas. DHRS2 downregulation was associated significantly with ESCC invasion, lymph nodes metastasis and clinical staging (P<0.001). DHRS2 could reduce reactive oxygen species and decrease nicotinamide adenine dinucleotide phosphate (oxidized/reduced), increase p53 stability and decrease Rb phosphorylation; it also decreased p38 mitogen-activated protein kinase phosphorylation and matrix metalloproteinase 2. That DHRS2 had an important part in ESCC development and progression.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,439 Da
NCBI Official Full Name
dehydrogenase/reductase SDR family member 2 isoform 2
NCBI Official Synonym Full Names
dehydrogenase/reductase (SDR family) member 2
NCBI Official Symbol
DHRS2
NCBI Official Synonym Symbols
HEP27; SDR25C1
NCBI Protein Information
dehydrogenase/reductase SDR family member 2; protein D; dicarbonyl reductase HEP27; dehydrogenase/reductase member 2; short-chain alcohol dehydrogenase family member; short chain dehydrogenase/reductase family 25C, member 1
UniProt Protein Name
Dehydrogenase/reductase SDR family member 2
UniProt Gene Name
DHRS2
UniProt Entry Name
DHRS2_HUMAN

Uniprot Description

DHRS2: Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2- propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. May inhibit cell replication. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.-; Oxidoreductase; EC 1.1.-.-

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nuclear envelope; nucleus

Molecular Function: carbonyl reductase (NADPH) activity

Biological Process: negative regulation of cell proliferation; response to toxin; C21-steroid hormone metabolic process; myeloid dendritic cell differentiation; negative regulation of apoptosis

Research Articles on DHRS2

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Product Notes

The DHRS2 dhrs2 (Catalog #AAA8101956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DHRS2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the DHRS2 dhrs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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