MGAT5 active protein

MGAT5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120802

• Gene Names: MGAT5; GNT-V; GNT-VA
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MGAT5; MGAT5 Protein; Human; Recombinant (His Tag); Human MGAT5/GGNT5 Protein (His Tag); GNT-V Protein; GNT-VA Protein; mannosyl (alpha-1; 6-)-glycoprotein beta-1; 6-N-acetyl-glucosaminyltransferase; MGAT5 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu189-Leu741

• Species: Human
• Activity: Measured by its ability to transfer N-Acetyl-alpha-D-glucosamine from UDP-N-Acetyl-alpha-D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >10pmoles/min/ug
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 189
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human MGAT5 (NP_002401.1) C-terminal segment (Leu 189-Leu 741) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MGAT5 active protein

Background: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, also known as Alpha-mannoside beta-1,6-N-acetylglucosaminyl-transferase, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, MGAT5, and GGNT5, is a single-pass type II membrane protein that belongs to the glycosyltransferase 18 family. MGAT5/GGNT5 catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The central nervous system (CNS) is rich in glycoconjugates, located on the cell surface and in the extracellular matrix. MGAT5/GGNT5 modification of complex-type N-glycans on CNS glycoproteins is involved in the regulation of depression-like behavior. Inhibitors of MGAT5/GGNT5 might be useful in the treatment of malignancies by targeting their dependency on focal adhesion signaling for growth and metastasis.

References

Morgan,R. et al., 2004,J Immunol 173 (12): 7200-8. Cheung,P. et al., 2007,Glycobiology 17 (7): 767-73. Li,D. et al., 2008, J Immunol 180 (5): 3158-65. Soleimani,L. et al., 2008, Genes Brain Behav. 7 (3):334-43.

NCBI and Uniprot Product Information

• NCBI GI #: 4505167
• NCBI GeneID: 4249
• NCBI Accession #: NP_002401.1
• NCBI GenBank Nucleotide #: NM_002410.4
• UniProt Accession #: Q09328; D3DP70
• Molecular Weight: 84,543 Da
• NCBI Official Full Name: alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• NCBI Official Synonym Full Names: mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
• NCBI Official Symbol: MGAT5
• NCBI Official Synonym Symbols: GNT-V; GNT-VA
• NCBI Protein Information: alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• UniProt Protein Name: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• Protein Family: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
• UniProt Gene Name: MGAT5
• UniProt Synonym Gene Names: GGNT5; GNT-V

NCBI Description

The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]

Uniprot Description

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.