Rabbit EB3 Polyclonal Antibody | anti-EB3 antibody

EB3 Polyclonal Antibody

Cat. Num. AAA3006734

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EB3; Polyclonal Antibody; EB3 Polyclonal Antibody; Microtubule-associated protein RP/EB family member 3; EB1 protein family member 3; EBF3; End-binding protein 3; RP3; MAPRE3; anti-EB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EB3 polyclonal antibody detects endogenous levels of EB3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 281

• Applicable Applications for anti-EB3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human EB3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EB3 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-EB3 antibody

EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL.

NCBI and Uniprot Product Information

• NCBI GI #: 10800412
• NCBI Accession #: NP_036458.2
• NCBI GenBank Nucleotide #: NP_036458.2
• UniProt Accession #: Q9UPY8
• Molecular Weight: ~ 32kDa
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 3
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 3
• UniProt Gene Name: MAPRE3
• UniProt Synonym Gene Names: EBF3; EB3
• UniProt Entry Name: MARE3_HUMAN

Uniprot Description

EB3: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. Belongs to the MAPRE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding

Chromosomal Location of Human Ortholog: 2p23.3-p23.1

Cellular Component: microtubule cytoskeleton; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; midbody

Molecular Function: protein binding; microtubule binding

Biological Process: mitosis; positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of transcription, DNA-dependent