Western Blot (WB)
(Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals CM5051 anti-CLASP2 (clone KT68) (lanes 1 & 2) and CM5071 anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.)
Rat CLASP2 Monoclonal Antibody | anti-CLASP2 antibody
CLASP2 (N-terminus) Antibody
ICC: 1:200
WB: 1:1000
Western Blot (WB)
(Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals CM5051 anti-CLASP2 (clone KT68) (lanes 1 & 2) and CM5071 anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.)
Western Blot (WB)
(Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals CM5051 anti-CLASP2 (clone KT68) (lanes 1 & 2) and CM5071 anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha-Tubulin (TM4111) (left) and rat monoclonal anti-CLASP2 (CM5051) (middle). The antibodies were detected using either goat anti-mouse:DyLight 488 (MS3011) or goat anti-Rat:DyLight 594 (RS3111).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha-Tubulin (TM4111) (left) and rat monoclonal anti-CLASP2 (CM5051) (middle). The antibodies were detected using either goat anti-mouse:DyLight 488 (MS3011) or goat anti-Rat:DyLight 594 (RS3111).)
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1-74.
NCBI and Uniprot Product Information
Uniprot Description
CLASP2: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. Belongs to the CLASP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Cellular Component: kinetochore; Golgi apparatus; microtubule; cell projection; cytoskeleton; membrane; cytoplasmic microtubule; cytoplasm; plasma membrane; cell cortex; trans-Golgi network; chromosome; chromosome, pericentric region
Molecular Function: microtubule plus-end binding; protein binding; microtubule binding
Biological Process: microtubule organizing center organization and biogenesis; regulation of microtubule-based process; mitosis; cell migration; meiotic cell cycle; cell division; negative regulation of microtubule depolymerization; establishment and/or maintenance of cell polarity; microtubule nucleation; cell cycle; regulation of microtubule polymerization or depolymerization
Research Articles on CLASP2
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Product Notes
The CLASP2 clasp2 (Catalog #AAA474268) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CLASP2 (N-terminus) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLASP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:200 WB: 1:1000. Researchers should empirically determine the suitability of the CLASP2 clasp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLASP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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