Rabbit EB3 Polyclonal Antibody | anti-EB3 antibody

EB3 (a.a. 171-182)

Cat. Num. AAA474101

• Gene Names: MAPRE3; EB3; RP3; EBF3; EBF3-S
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: EB3; Polyclonal Antibody; EB3 (a.a. 171-182); Microtubule RP/EB; MAPRE3; APC; End-binding RP3; anti-EB3 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using EB3 (a.a. 171-182) peptide (without carrier). The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of EB3 on SDS-PAGE immunoblots of mouse brain and human recombinant full length EB3 protein.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 281

• Applicable Applications for anti-EB3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000; WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
• Immunogen: EB3 (a.a. 171-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues 171 to 182 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and has low homology to other EB family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8). )

Related Product Information for anti-EB3 antibody

Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.

References

Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334.Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1.Komarova, Y. et al. (2012) Mol Cell. 48:914.

NCBI and Uniprot Product Information

• NCBI GI #: 10800412
• NCBI GeneID: 22924
• NCBI Accession #: NP_036458.2
• NCBI GenBank Nucleotide #: NM_012326.3
• UniProt Accession #: Q9UPY8
• Molecular Weight: 32 kDa
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 3
• NCBI Official Synonym Full Names: microtubule associated protein RP/EB family member 3
• NCBI Official Symbol: MAPRE3
• NCBI Official Synonym Symbols: EB3; RP3; EBF3; EBF3-S
• NCBI Protein Information: microtubule-associated protein RP/EB family member 3
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 3
• UniProt Gene Name: MAPRE3
• UniProt Synonym Gene Names: EBF3; EB3
• UniProt Entry Name: MARE3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EB3: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. Belongs to the MAPRE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding

Chromosomal Location of Human Ortholog: 2p23.3-p23.1

Cellular Component: microtubule cytoskeleton; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; midbody

Molecular Function: protein binding; microtubule binding

Biological Process: mitosis; positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of transcription, DNA-dependent