Rabbit anti-Human, Mouse ATAD3A Polyclonal Antibody | anti-ATAD3A antibody

Rabbit anti-human ATAD3A polyclonal Antibody

Cat. Num. AAA1491844

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: ATAD3A; Polyclonal Antibody; Rabbit anti-human ATAD3A polyclonal Antibody; ATPase family AAA domain-containing protein 3A; anti-ATAD3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 586

• Applicable Applications for anti-ATAD3A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB:1:500-5000; IHC:1:20-1:200; Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human ATPase family AAA domain-containing protein 3A protein (1-240aa)
• Immunogen Species: Homo Sapiens (Human)
• Research Area: Cell Biology
• Target Names: ASAD3A
• Preparation and Storage: Upon receipt, aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: ATAD3A antibody at 4ug/mlLane 1: A549 whole cell lysateLane 2: Mouse spleen tissueLane 3: Mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 72,67,58 kDaObserved band size: 71 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1491844 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1491844 at dilution 1:100)

Related Product Information for anti-ATAD3A antibody

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 283436222
• NCBI GeneID: 55210
• NCBI Accession #: NP_001164006.1
• NCBI GenBank Nucleotide #: NM_001170535.1
• UniProt Accession #: Q9NVI7; Q5SV23; Q8N275; Q96A50; B3KPB3; D2K8Q1; G3V1I6
• NCBI Official Full Name: ATPase family AAA domain-containing protein 3A isoform 2
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 3A
• NCBI Official Symbol: ATAD3A
• NCBI Protein Information: ATPase family AAA domain-containing protein 3A
• UniProt Protein Name: ATPase family AAA domain-containing protein 3A
• Protein Family: ATPase family AAA domain-containing protein
• UniProt Gene Name: ATAD3A
• UniProt Entry Name: ATD3A_HUMAN

NCBI Description

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM, Sep 2008]

Uniprot Description

ATAD3A: Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone- dependent steroidogenesis. Belongs to the AAA ATPase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: ATP binding

Biological Process: cell growth; negative regulation of apoptosis