Mouse BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD Monoclonal Antibody | anti-BTAF1 antibody

BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170)

Cat. Num. AAA603609

• Gene Names: BTAF1; MOT1; TAF172; KIAA0940; TAFII170; MGC138406; TAF(II)170
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: BTAF1 RNA Polymerase II; B-TFIID Transcription Factor-Associated; 170kD; Monoclonal Antibody; 170kD (BTAF1; KIAA0940; Mot1 Homolog (S. cerevisiae); TAF172; TAFII170; TAF(II)170); Anti -BTAF1 RNA Polymerase II; anti-BTAF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C46
• Specificity: Recognizes human BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-BTAF1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.; Dilution: Flow Cytometry: 0.5-2ug/ml; Western Blot: 0.5-2ug/ml; Immunocytochemistry: 2-50ug/ml
• Immunogen: Partial sequence of recombinant full-length protein to human BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Western Blot (WB)

(WB: Detection of BTAF1 by Western Blot. Samples: Whole cell lysate from human HeLa (H, 50ug), mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells. Predicted molecular weight: 206kD.)

Testing Data

(FC: HeLA cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS603609 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG.)

Immunoprecipitation (IP)

(IP: RIPA lysate of HeLa cells was incubated with MBS603609.)

Immunostaining (IS)

(IC: Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS603609.)

Related Product Information for anti-BTAF1 antibody

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.

Product Categories/Family for anti-BTAF1 antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 119570488
• NCBI GeneID: 9044
• UniProt Accession #: O14981; O43578; Q05C17; Q59HB6; Q6PI32; Q8N6J1
• Molecular Weight: 206,887 Da
• NCBI Official Full Name: BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), isoform CRA_a
• NCBI Official Synonym Full Names: BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)
• NCBI Official Symbol: BTAF1
• NCBI Official Synonym Symbols: MOT1; TAF172; KIAA0940; TAFII170; MGC138406; TAF(II)170
• NCBI Protein Information: TATA-binding protein-associated factor 172; OTTHUMP00000020093; TBP-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit
• UniProt Protein Name: TATA-binding protein-associated factor 172
• Protein Family: TATA-binding protein-associated factor
• UniProt Gene Name: BTAF1
• UniProt Synonym Gene Names: TAF172
• UniProt Entry Name: BTAF1_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM]

Uniprot Description

TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family.

Protein type: Transcription initiation complex; EC 3.6.1.-; EC 3.6.4.-; Helicase

Chromosomal Location of Human Ortholog: 10q22-q23

Cellular Component: nucleus

Molecular Function: DNA binding; helicase activity; transcription factor activity; ATP binding

Biological Process: metabolic process; negative regulation of transcription, DNA-dependent