Flow Cytometry (FC/FACS)
(Figure: FACS analysis of cells with MBS603412.|HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse DcTRAIL-R2 (thick line). Cells (5x10e5) were incubated on ice for 30 minutes in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MBS603412. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 minutes, washed and then analyzed by Flow Cytometry.)
Rat anti-Mouse DcTRAIL-R2 Monoclonal Antibody
DcTRAIL-R2 (Decoy TRAIL Receptor 2)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Highly PurifiedPurified from concentrated hybridoma tissue culture supernatant 95% (SDS-PAGE)
Synonyms
DcTRAIL-R2; Monoclonal Antibody; DcTRAIL-R2 (Decoy TRAIL Receptor 2); Anti -DcTRAIL-R2 (Decoy TRAIL Receptor 2); anti-DcTRAIL-R2 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6D350
Specificity
Recognizes mouse DcTRAIL-R2. Does not cross-react with mouse DcTRAIL-R1.
Purity/Purification
Highly Purified
Purified from concentrated hybridoma tissue culture supernatant 95% (SDS-PAGE)
Purified from concentrated hybridoma tissue culture supernatant 95% (SDS-PAGE)
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for anti-DcTRAIL-R2 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry. Not suitable for use in Immunoprecipitation.
Dilution: Flow Cytometry: 1:1000
Optimal dilution to be determined by researcher.
Dilution: Flow Cytometry: 1:1000
Optimal dilution to be determined by researcher.
Immunogen
Recombinant mouse DcTRAIL-R2:Fc. The extracellular domain of mouse DcTRAIL-R2 (aa 1-170).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Flow Cytometry (FC/FACS)
(Figure: FACS analysis of cells with MBS603412.|HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse DcTRAIL-R2 (thick line). Cells (5x10e5) were incubated on ice for 30 minutes in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MBS603412. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 minutes, washed and then analyzed by Flow Cytometry.)
Flow Cytometry (FC/FACS)
(Figure: FACS analysis of cells with MBS603412.|HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse DcTRAIL-R2 (thick line). Cells (5x10e5) were incubated on ice for 30 minutes in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MBS603412. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 minutes, washed and then analyzed by Flow Cytometry.)
Related Product Information for anti-DcTRAIL-R2 antibody
Decoy receptor 2 (DcR2) is one of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors and suppresses TRAIL-induced apoptosis. Its expression, like the other three TRAIL receptors (i.e., DR4, DR5, and DcR1), is regulated by p53. DcR2 is a p53 target gene and regulates chemosensitivity. p53 directly regulates the DcR2 gene expression via an intronic p53BS. In addition, overexpression of DcR2 conferred resistance to TRAIL-mediated apoptosis and attenuated cell response to DNA-damaging agents, whereas silencing of DcR2 expression enhanced chemotherapeutic agent-induced apoptosis. These results suggest that DcR2 regulates chemosensitivity.
Product Categories/Family for anti-DcTRAIL-R2 antibody
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Product Notes
The DcTRAIL-R2 (Catalog #AAA603412) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The DcTRAIL-R2 (Decoy TRAIL Receptor 2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DcTRAIL-R2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Not suitable for use in Immunoprecipitation. Dilution: Flow Cytometry: 1:1000 Optimal dilution to be determined by researcher. Researchers should empirically determine the suitability of the DcTRAIL-R2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DcTRAIL-R2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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