Testing Data
(Figure: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid coding for the extracellular domain of human DR6 (thick line).Method: Cells (5x105) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1ug/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.)
anti-Human DR6 Monoclonal Antibody | anti-Death receptor-6; TNFRSF 21 antibody
DR6 (human) monoclonal antibody (Luke-1)
Short term: 4 degree C
Avoid freeze/thaw cycles. Stable for at least 1 year after receipt when stored as recommended.
Testing Data
(Figure: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid coding for the extracellular domain of human DR6 (thick line).Method: Cells (5x105) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1ug/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.)
Testing Data
(Figure: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid coding for the extracellular domain of human DR6 (thick line).Method: Cells (5x105) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1ug/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation. [provided by RefSeq, Jul 2013]
Uniprot Description
DR6: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N- terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Associates with TRADD. Interacts with N-APP. Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.
Protein type: Membrane protein, integral; Receptor, cytokine; Apoptosis
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: axon; integral to plasma membrane; intrinsic to plasma membrane; plasma membrane
Molecular Function: protein binding; tumor necrosis factor receptor activity
Biological Process: adaptive immune response; apoptosis; B cell apoptosis; cellular lipid metabolic process; humoral immune response; immune response; inflammatory response; myelination; negative regulation of B cell proliferation; negative regulation of myelination; negative regulation of T cell proliferation; neuron apoptosis; regulation of apoptosis; regulation of cell proliferation; regulation of oligodendrocyte differentiation; response to lipopolysaccharide; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway
Research Articles on Death receptor-6; TNFRSF 21
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Product Notes
The Death receptor-6; TNFRSF 21 tnfrsf21 (Catalog #AAA565147) is an Antibody produced from Rat: Purified from concentrated hybridoma tissue culture supernatant. and is intended for research purposes only. The product is available for immediate purchase. The DR6 (human) monoclonal antibody (Luke-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DR6 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the Death receptor-6; TNFRSF 21 tnfrsf21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DR6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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