Flow Cytometry (FC/FACS)
(Figure: FACS analysis of cells with MAb to LTbR (human) (Carlos-1) .Method: HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of human LTbR (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to LTbR (human) (Carlos-1) . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.)
anti-Human LT beta R Monoclonal Antibody | anti-LT beta R antibody
LT beta R (human) monoclonal antibody (Carlos-1)
Immunoprecipitation (1:200)
Suggested dilutions/conditions may not be available for all applications.
Short term: 4 degree C
Avoid freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Figure: FACS analysis of cells with MAb to LTbR (human) (Carlos-1) .Method: HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of human LTbR (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to LTbR (human) (Carlos-1) . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Figure: FACS analysis of cells with MAb to LTbR (human) (Carlos-1) .Method: HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of human LTbR (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to LTbR (human) (Carlos-1) . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012]
Uniprot Description
LTBR: Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: identical protein binding; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding
Biological Process: immune response; inflammatory response; multicellular organismal development; myeloid dendritic cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of JNK cascade; regulation of cell proliferation; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway; viral reproduction
Research Articles on LT beta R
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Product Notes
The LT beta R ltbr (Catalog #AAA565141) is an Antibody produced from Rat: Purified from concentrated hybridoma tissue culture supernatant. and is intended for research purposes only. The product is available for immediate purchase. The LT beta R (human) monoclonal antibody (Carlos-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LT beta R can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry (1:1,000) Immunoprecipitation (1:200) Suggested dilutions/conditions may not be available for all applications. Researchers should empirically determine the suitability of the LT beta R ltbr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LT beta R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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