Rabbit Cyclin B1 Monoclonal Antibody | anti-CCNB1 antibody

Cyclin B1, phosphorylated (Ser133)

Cat. Num. AAA609069

• Gene Names: CCNB1; CCNB
• Applications: Western Blot, Immunoprecipitation
• Purity: Supernatant; Supernatant
• Synonyms: Cyclin B1; Monoclonal Antibody; phosphorylated (Ser133); Anti -Cyclin B1; anti-CCNB1 antibody

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 8A11
• Specificity: Recognizes endogenous levels of human cyclin B1 protein only when phosphorylated at Ser133 at ~58kD.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CCNB1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:100
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser133 of human cyclin B1, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from HeLa cells, treated with hydroxyurea or nocodazole for 24 hours, using MBS609069 (upper) or Cyclin B1 (lower).)

Related Product Information for anti-CCNB1 antibody

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15. The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events. Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint. Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation. While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well.

Product Categories/Family for anti-CCNB1 antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 13518072
• NCBI GeneID: 891
• UniProt Accession #: Q9BPX9; P14635; Q5TZP9; Q9BWU7; Q9BWU8; Q9BWU9; Q9BWV0
• Molecular Weight: 13,039 Da
• NCBI Official Full Name: cyclin B1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1; OTTHUMP00000123478; OTTHUMP00000221981; OTTHUMP00000223023; G2/mitotic-specific cyclin B1
• UniProt Protein Name: Cyclin B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Entry Name: Q9BPX9_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; histone kinase activity; patched binding; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; tissue regeneration; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; response to mechanical stimulus; spermatogenesis; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; mitotic spindle stabilization; G1/S transition of mitotic cell cycle