Rabbit anti-Human CCNB1 Polyclonal Antibody | anti-CCNB1 antibody

CCNB1 Antibody

Cat. Num. AAA9410400

• Gene Names: CCNB1; CCNB
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: CCNB1; Polyclonal Antibody; CCNB1 Antibody; CCNB; cyclinB1; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total CCNB1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 433

• Applicable Applications for anti-CCNB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:50 - 1:200; Immunoprecipitation: 1:20 - 1:100
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human CCNB1.
• Target Name: CCNB1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CCNB1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using CCNB1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-CCNB1 antibody

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).

Product Categories/Family for anti-CCNB1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14327896
• NCBI GeneID: 891
• NCBI Accession #: NP_114172.1
• NCBI GenBank Nucleotide #: NM_031966.3
• UniProt Accession #: P14635; Q5TZP9; A8K066
• Molecular Weight: 44,102 Da
• NCBI Official Full Name: G2/mitotic-specific cyclin-B1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1
• UniProt Protein Name: G2/mitotic-specific cyclin-B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB1
• UniProt Synonym Gene Names: CCNB
• UniProt Entry Name: CCNB1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; patched binding; histone kinase activity; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; tissue regeneration; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; mitotic metaphase plate congression; oocyte maturation; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle