Rabbit CCNB1 Antibody | anti-CCNB1 antibody

CCNB1 Antibody

Cat. Num. AAA8502870

• Gene Names: CCNB1; CCNB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: CCNB1; CCNB1 Antibody; CCNB; cyclin B1; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 433

• Applicable Applications for anti-CCNB1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide of human CCNB1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of HepG2cell lines, using CCNB1 antibody.)

Related Product Information for anti-CCNB1 antibody

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1, 2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6, 10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).

Product Categories/Family for anti-CCNB1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90. 2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9. 3. Norbury, C. et al. (1991) EMBO J 10, 3321-9. 4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. 5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001. 6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20. 7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7. 8. Hagting, A. et al. (1999) Curr Biol 9, 680-9. 9. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65. 10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8. 11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61. 12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63. 13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23. 14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

NCBI and Uniprot Product Information

• NCBI GI #: 14327896
• NCBI GeneID: 891
• NCBI Accession #: NP_114172.1
• NCBI GenBank Nucleotide #: NM_031966.3
• UniProt Accession #: P14635; Q5TZP9; A8K066
• Molecular Weight: 48kDa
• NCBI Official Full Name: G2/mitotic-specific cyclin-B1 isoform 1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1
• UniProt Protein Name: G2/mitotic-specific cyclin-B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB1
• UniProt Synonym Gene Names: CCNB

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]

Uniprot Description

Essential for the control of the cell cycle at the G2/M (mitosis) transition.