Western Blot (WB)
(Western blot analysis of extracts from hydroxyurea-treated HT29 cells, aphidicolin-treated HeLa cells, and asynchronous and mitotic CHO cells using MBS623820)
Rabbit cdc2 Polyclonal Antibody | anti-cdc2 antibody
cdc2, phosphorylated (Thr14)
Gene Names
cdc2; 5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
Reactivity
Hamster, Human, Monkey
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
cdc2; Polyclonal Antibody; phosphorylated (Thr14); Anti -cdc2; anti-cdc2 antibody
Host
Rabbit
Reactivity
Hamster, Human, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of human cdc2 (CDK1) only when phosphorylated at Thr14. Species crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Applicable Applications for anti-cdc2 antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr14 of human cdc2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from hydroxyurea-treated HT29 cells, aphidicolin-treated HeLa cells, and asynchronous and mitotic CHO cells using MBS623820)
Western Blot (WB)
(Western blot analysis of extracts from hydroxyurea-treated HT29 cells, aphidicolin-treated HeLa cells, and asynchronous and mitotic CHO cells using MBS623820)
Western Blot (WB)
(Western blot analysis of extracts from asynchronous and mitotic HeLa cells using MBS623820)
Western Blot (WB)
(Western blot analysis of extracts from asynchronous and mitotic HeLa cells using MBS623820)
Related Product Information for anti-cdc2 antibody
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (2). Phosphorylation at Thr14 and Tyr15 resulting in inhibition of cdc2 can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
Product Categories/Family for anti-cdc2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cdc2
NCBI Official Symbol
cdc2
NCBI Official Synonym Symbols
5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
NCBI Protein Information
CG5363 gene product from transcript CG5363-RA; cdc2; CG5363-PA; cdc2-PA; cyclin-dependent kinase 1; CDC2 kinase; cyclin dependent kinase
Protein Family
Research Articles on cdc2
Similar Products
Product Notes
The cdc2 (Catalog #AAA623820) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The cdc2, phosphorylated (Thr14) reacts with Hamster, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's cdc2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the cdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cdc2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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