Rabbit anti-Human Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody

Cyclin B1, phosphorylated (Ser147)

Cat. Num. AAA625299

• Gene Names: CCNB1; CCNB
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography
• Synonyms: Cyclin B1; Polyclonal Antibody; phosphorylated (Ser147); Anti -Cyclin B1; anti-CCNB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous cyclin B1 only when phosphorylated on serine147. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol

• Applicable Applications for anti-CCNB1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phospho-Ser147 peptide corresponding to residues surrounding serine 147 of human cyclin B1 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of 293 cell extracts using MBS625299(A) and Cyclin B1 (V152)(C). Incubation of the nitrocellulose membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer abolishes the phospho-cyclin B1 signal (B), but has no effect on the total cyclin B1 signal (D).)

Western Blot (WB)

(Western blot analysis of lysates from asynchronous cells (lane 1), S-phase and G2/M blocked cells (lane 2), or synchronized cells collected at various timepoints following release from this block (lanes 3-7) using MBS625299(upper). Cell cycle synchronization was verified by flow cytometric analysis of DNA content (lower). (Data kindly provided by Ethan Kohn and Alan Eastman, Dartmouth Medical School, Hanover, NH).)

Testing Data

Related Product Information for anti-CCNB1 antibody

Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at threonine 161, and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of cdk inhibitors and the cdk-associated cyclins are regulated by phosphorylation, ubiquitination and degradation allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 has been shown to be required for cdc25C-dependent dephosphorylation of cdc2 Tyr15 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 has been shown to phosphorylate cyclin B1 at Ser147 (8,12).

Product Categories/Family for anti-CCNB1 antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 13518072
• NCBI GeneID: 891
• UniProt Accession #: Q9BPX9; P14635; Q5TZP9; Q9BWU7; Q9BWU8; Q9BWU9; Q9BWV0
• Molecular Weight: 13,039 Da
• NCBI Official Full Name: cyclin B1
• NCBI Official Synonym Full Names: cyclin B1
• NCBI Official Symbol: CCNB1
• NCBI Official Synonym Symbols: CCNB
• NCBI Protein Information: G2/mitotic-specific cyclin-B1; OTTHUMP00000123478; OTTHUMP00000221981; OTTHUMP00000223023; G2/mitotic-specific cyclin B1
• UniProt Protein Name: Cyclin B1
• Protein Family: G2/mitotic-specific cyclin
• UniProt Entry Name: Q9BPX9_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Uniprot Description

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; patched binding; histone kinase activity; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; mitotic nuclear envelope disassembly; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; tissue regeneration; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; gut development; cell division; response to mechanical stimulus; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle