Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Rat anti-Mouse CD31 Monoclonal Antibody | anti-CD31 antibody
RAT ANTI MOUSE CD31
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence
Synonyms
CD31; Monoclonal Antibody; RAT ANTI MOUSE CD31; CD31 antibody|ER-MP12; anti-CD31 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ER-MP12
Specificity
CD31
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-CD31 antibody
Immunohistochemistry (IHC)-Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence
Application Notes
FC/FACS: Min Dilution: 1/50 (Pack Size: 25ug, 0.25mg), 1/10 (Pack Size: 0.1mg); Max Dilution: 1/200 (Pack Size: 25ug, 0.25mg), 1/100 (Pack Size: 0.1mg)
(IHC)-Frozen 1: 1/10-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(IHC)-Frozen 1: 1/10-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunogen
BALB/c macrophage precursor cell hybrids
Preservative Stabilizers
0.09% Sodium Azide
Fusion Partners
Cells from immunised rats were fused with the cells of the rat Y3-Ag1.2.3 myeloma cell line
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Testing Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Related Product Information for anti-CD31 antibody
Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein that is expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.
CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).
CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).
References
1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.
2. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.Am J Pathol. 158 (1): 131-45.
3. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.
4. Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.Arterioscler Thromb Vasc Biol. 30: 909-14.
5. Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.J Lipid Res. 52 (5): 1033-8.
2. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.Am J Pathol. 158 (1): 131-45.
3. de Bruijn, M.F. et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.
4. Revermann, M. et al. (2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.Arterioscler Thromb Vasc Biol. 30: 909-14.
5. Thorp, E. et al. (2011) A reporter for tracking the UPR in vivo reveals patterns of temporal and cellular stress during atherosclerotic progression.J Lipid Res. 52 (5): 1033-8.
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Product Notes
The CD31 (Catalog #AAA225965) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI MOUSE CD31 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC)-Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence. FC/FACS: Min Dilution: 1/50 (Pack Size: 25ug, 0.25mg), 1/10 (Pack Size: 0.1mg); Max Dilution: 1/200 (Pack Size: 25ug, 0.25mg), 1/100 (Pack Size: 0.1mg) (IHC)-Frozen 1: 1/10-1/100 FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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