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Testing Data
(Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE (MBS216020))
Rat Ly-6C Monoclonal Antibody | anti-Ly-6C antibody
RAT ANTI MOUSE Ly-6C
Gene Names
Ly6c2; Ly-6C2; Ly-6C.2
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
Ly-6C; Monoclonal Antibody; RAT ANTI MOUSE Ly-6C; anti-Ly-6C antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ER-MP20
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-Ly-6C antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE (MBS216020))
Testing Data
(Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE (MBS216020))
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MBS216014) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MBS216014) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MBS216014) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MBS216014) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MBS216014) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 (MBS216014) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)
Related Product Information for anti-Ly-6C antibody
Rat anti Mouse Ly-6C monoclonal antibody, clone ER-MP20 recognizes murine Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state. Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).
NCBI and Uniprot Product Information
Uniprot Description
LY6C2:
Cellular Component: membrane; plasma membrane
Similar Products
Product Notes
The Ly-6C (Catalog #AAA216022) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ly-6C can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the Ly-6C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly-6C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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