Arginase II Recombinant Protein | Arg2 recombinant protein

Recombinant Arginase II (Arg2)

Cat. Num. AAA2030357

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Arginase II; Recombinant Arginase II (Arg2); Arg2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; VHSVAVIG APFSQGQKRK GVEYGPAAVR XAGLMKRLSD LGCHLKDFGD LNFTPVPKDD LYNNLIVNPR SVGLANQELA EVVSRAVSGG YSCVTVGGDH SLAIGTISGH ARHCPDLGVI WVDAHADINT PLTTSSGNLH GQPVSFLLRE LQDKVPQLPG FSWIKPCISS PSIVYIGLRD VDPPEHFILK NYDIQYFSMR DIDRLGIQKV MEQTFDLLIG KRQRPIHLSF DIDAFDPTLA PATGTPVVGG LTYREGIYIT EEIHSTGLLS ALDLVEVNPR LAVSEEEAKA TASLAVDVIASSFGQTREGG HIVYDQLPTP SSPDESESEE RVRI
• Sequence Length: 354

• Applicable Applications for Arg2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 39.6KDa
• Accurate Molecular Mass (KD): 43kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Bos taurus; Bovine (Cattle)
• Fragment: Val23~Ile354
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 1763758
• NCBI GeneID: 384
• Molecular Weight: 38,578 Da
• NCBI Official Full Name: arginase II
• NCBI Official Synonym Full Names: arginase 2
• NCBI Official Symbol: ARG2
• NCBI Protein Information: arginase-2, mitochondrial
• UniProt Protein Name: Arginase-2, mitochondrial
• Protein Family: Arginase
• UniProt Gene Name: ARG2
• UniProt Entry Name: ARGI2_HUMAN

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ARG2: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. Belongs to the arginase family.

Protein type: EC 3.5.3.1; Amino Acid Metabolism - arginine and proline; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: metal ion binding; arginase activity

Biological Process: striated muscle contraction; arginine metabolic process; ureteric bud development; nitric oxide biosynthetic process; urea cycle