Testing Data
(Jurkat cells were grown in suspension to 4 x 10^5 cells/mL, then divided into two separate TC-flasks. One population, "Non-Induced", received a DMSO vehicle control (A). The other population, "Induced", was spiked with 1 muM staurosporine (B). Cells were incubated for 4 hours at 37 degree C, then stained with red SR-FLICA caspase-8 inhibitor, SR-LETD-FMK (kit catalog #9150) for 1 hour at 37 degree C. After labeling, samples were washed three times and slides were prepared. Fluorescence images were acquired using a Nikon Eclipse 90i microscope equipped with a Hamamatsu Flash 4.0 camera. In the treated sample, cells appear bright red, indicating a high level of caspase-8 activity (B, Induced, right). In the non-induced sample, few red positive cells are visible, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy (220:68, 121815).)
SR FLICA Caspase 8 Assay Kit
SR FLICA Caspase 8 Assay Kit
Synonyms
SR FLICA Caspase 8; SR FLICA Caspase 8 Assay Kit; SR FLICA Caspase 8 assay kit
Samples
Cell culture
Target
Caspase-8
Excitation/Emission
550-580 nm / 590-600 nm
Reagent
SR-LETD-FMK
Reagent Name
SR-LETD-FMK
Preparation and Storage
Store at 2-8 degree C.
Testing Data
(Jurkat cells were grown in suspension to 4 x 10^5 cells/mL, then divided into two separate TC-flasks. One population, "Non-Induced", received a DMSO vehicle control (A). The other population, "Induced", was spiked with 1 muM staurosporine (B). Cells were incubated for 4 hours at 37 degree C, then stained with red SR-FLICA caspase-8 inhibitor, SR-LETD-FMK (kit catalog #9150) for 1 hour at 37 degree C. After labeling, samples were washed three times and slides were prepared. Fluorescence images were acquired using a Nikon Eclipse 90i microscope equipped with a Hamamatsu Flash 4.0 camera. In the treated sample, cells appear bright red, indicating a high level of caspase-8 activity (B, Induced, right). In the non-induced sample, few red positive cells are visible, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy (220:68, 121815).)
Testing Data
(Jurkat cells were grown in suspension to 4 x 10^5 cells/mL, then divided into two separate TC-flasks. One population, "Non-Induced", received a DMSO vehicle control (A). The other population, "Induced", was spiked with 1 muM staurosporine (B). Cells were incubated for 4 hours at 37 degree C, then stained with red SR-FLICA caspase-8 inhibitor, SR-LETD-FMK (kit catalog #9150) for 1 hour at 37 degree C. After labeling, samples were washed three times and slides were prepared. Fluorescence images were acquired using a Nikon Eclipse 90i microscope equipped with a Hamamatsu Flash 4.0 camera. In the treated sample, cells appear bright red, indicating a high level of caspase-8 activity (B, Induced, right). In the non-induced sample, few red positive cells are visible, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy (220:68, 121815).)
Related Product Information for SR FLICA Caspase 8 assay kit
Description: Detect caspase-8 activity with the SR-FLICA Caspase-8 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe SR-LETD-FMK to label active caspase-8 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
Background: Caspases play important roles in apoptosis and inflammation. The FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FLICA reagent SR-LETD-FMK enters each cell and irreversibly binds to activated caspase-8. Because the SR-LETD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound SR-LETD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the active caspase-8 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Hoechst 33342 to detect changes in nuclear morphology.
Background: Caspases play important roles in apoptosis and inflammation. The FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FLICA reagent SR-LETD-FMK enters each cell and irreversibly binds to activated caspase-8. Because the SR-LETD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound SR-LETD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the active caspase-8 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Hoechst 33342 to detect changes in nuclear morphology.
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Product Notes
The SR FLICA Caspase 8 (Catalog #AAA258048) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SR FLICA Caspase 8, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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