Human Chitinase domain-containing protein 1 ELISA Kit | CHID1 elisa kit

Human Chitinase domain-containing protein 1 ELISA Kit

Cat. Num. AAA764700

• Gene Names: CHID1; GL008; SICLP; SI-CLP
• Reactivity: Human
• Synonyms: Chitinase domain-containing protein 1; Human Chitinase domain-containing protein 1 ELISA Kit; CHID1/Chitinase domain-containing protein 1/Stabilin-1-interacting chitinase-like protein (SI-CLP); CHID1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHID1. No significant cross-reactivity or interference between CHID1 and analogues was observed.
• Sequence Length: 393

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHID1 elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 218083215
• NCBI GeneID: 66005
• NCBI Accession #: NP_001136147.1
• NCBI GenBank Nucleotide #: NM_001142675.1
• Molecular Weight: 41,678 Da
• NCBI Official Full Name: chitinase domain-containing protein 1 isoform a
• NCBI Official Synonym Full Names: chitinase domain containing 1
• NCBI Official Symbol: CHID1
• NCBI Official Synonym Symbols: GL008; SICLP; SI-CLP
• NCBI Protein Information: chitinase domain-containing protein 1
• UniProt Protein Name: Chitinase domain-containing protein 1
• Protein Family: Chitinase domain-containing protein
• UniProt Gene Name: CHID1
• UniProt Synonym Gene Names: SI-CLP
• UniProt Entry Name: CHID1_HUMAN

Uniprot Description

CHID1: Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro). Belongs to the glycosyl hydrolase 18 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; late endosome; lysosome; membrane; nucleus; trans-Golgi network

Molecular Function: chitin binding; chitinase activity; protein binding

Biological Process: carbohydrate metabolic process; chitin catabolic process; innate immune response