Testing Data
Mouse anti-Human CD137L Monoclonal Antibody | anti-CD137L antibody
anti-human CD137L-Purified Preservative Free
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Preservative Free
Synonyms
CD137L; Monoclonal Antibody; anti-human CD137L-Purified Preservative Free; Monoclonal anti-human CD137 L (4-1BB Ligand); anti-CD137L antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Murine IgG2a, kappa
Clone Number
ANC5D6
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD137L antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Performance
Five x 10^5 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 ul of anti-CD137L antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC,MBS666783 after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.29 log10 fluorescent units when compared to a Mouse IgG2a negative control MBS666112 at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Recombinant human CD137L
Preparation and Storage
Store at 2 - 5°C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended
Product should retain activity for at least 6 months after shipping date when stored as recommended
Testing Data
Testing Data
Related Product Information for anti-CD137L antibody
Information: Human CD137 Ligand is a type II transmembrane protein constitutively expressed by monocytes, B cells neuroblastoma cells (1), and some activated T cells. The interaction of CD137 (4-1BB) and CD137L can supply a costimulatory signal to T cells through CD137. This costimulus seems to be more effective for CD8+ effector T cells than for CD4+ helper T cells (3). The interaction can also effect a reverse signal, inducing monocyte activation (2), or T cell inactivation and programmed death (4) through CD137L. Anti-CD137L antibody binds to CD137L present on Raji and U-937 cells, and also binds to recombinant CD137L-muCD8 in EIA. This antibody does not block the interaction between CD137 and CD137L.
References
1) M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227. 2) J. Langstein, et al, (1998) J Immunol 160: 2488-2494. 3) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 4) H. Schwartz, et al, (1996) Blood 87: 2839. 5) G. Zhu, et al, (2001) J Immunol 167: 2671-6.
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Product Notes
The CD137L (Catalog #AAA666242) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD137L-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD137L can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the CD137L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD137L, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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