Testing Data
Mouse anti-Human CD252 Monoclonal Antibody | anti-CD252 antibody
anti-human CD252(OX40L)-Purified Preservative Free
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified Preservative Free
Synonyms
CD252; Monoclonal Antibody; anti-human CD252(OX40L)-Purified Preservative Free; Monoclonal anti-human CD252 (CD134L; OX40L); anti-CD252 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
ANC10G1
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD252 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Blocking
Performance
Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD134L antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 14% subpopulation of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human HUVEC and Recombinant soluble human CD134L
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD252 antibody
Information: Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of inflammation and rejection (5,6,7,8). Antibody from clone ANC10G1 binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.
References
1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2) L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. 4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 5) V. Malmstrom, et al, (2001) J Immunol 166: 6972. 6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115. 7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955. 8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.
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Product Notes
The CD252 (Catalog #AAA666578) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD252(OX40L)-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD252 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Blocking. Researchers should empirically determine the suitability of the CD252 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD252, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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