Human Ataxin 10 (ATXN10) ELISA Kit | ATXN10 elisa kit

Human Ataxin 10 (ATXN10) ELISA Kit

Cat. Num. AAA2705002

• Gene Names: ATXN10; E46L; SCA10; HUMEEP
• Reactivity: Human
• Synonyms: Ataxin 10 (ATXN10); Human Ataxin 10 (ATXN10) ELISA Kit; E46L; SCA10; Spinocerebellar Ataxia 10; Brain protein E46 homolog; ATXN10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ataxin 10 (ATXN10).; No significant cross-reactivity or interference between Ataxin 10 (ATXN10) and analogues was observed.
• Sequence Length: 411

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ataxin 10 (ATXN10) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ataxin 10 (ATXN10) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATXN10 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxin 10 (ATXN10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ataxin 10 (ATXN10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ataxin 10 (ATXN10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ataxin 10 (ATXN10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13960135
• NCBI GeneID: 25814
• Molecular Weight: 46,286 Da
• NCBI Official Full Name: Ataxin 10
• NCBI Official Synonym Full Names: ataxin 10
• NCBI Official Symbol: ATXN10
• NCBI Official Synonym Symbols: E46L; SCA10; HUMEEP
• NCBI Protein Information: ataxin-10
• UniProt Protein Name: Ataxin-10
• Protein Family: Ataxin
• UniProt Gene Name: ATXN10
• UniProt Synonym Gene Names: SCA10

NCBI Description

This gene encodes a protein that may function in neuron survival, neuron differentiation, and neuritogenesis. These roles may be carried out via activation of the mitogen-activated protein kinase cascade. Expansion of an ATTCT repeat from 9-32 copies to 800-4500 copies in an intronic region of this locus has been associated with spinocerebellar ataxia, type 10. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jul 2016]

Uniprot Description

Necessary for the survival of cerebellar neurons. Induces neuritogenesis by activating the Ras-MAP kinase pathway. May play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.