Testing Data
CD137 protein
CD137(4-1BB)-huIg-PE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Synonyms
CD137; CD137(4-1BB)-huIg-PE; Human CD137(4-1BB) huIg/R-PE Fusion Protein; CD137 protein
Host
CHO cells
Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 200 ul (varies by lot)
Sequence Length
41
Applicable Applications for CD137 protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
1:20 (or use 4 ul of concentrated stock per 5 x 105-cell test)
Molecular Structure
A soluble 55 kd fusion protein consisting of the extracellular (186aa) domain of human CD137 (4-1BB) fused to human IgG1 Fc (4).
Transfectant Cell Line
CHO
Performance
Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul of CD137-huIg/R-PE at a dilution factor of 1:20 (10 ug/ml). Cells were washed three times, fixed and analyzed using by FACS. The cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to a buffer background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of a mixture of 0.5 mg/ml unlabeled CD137-huIg.
Conjugation
PE
Production
Human CD137 Ig fusion protein was purified by Protein A and size exclusion chromatography from (low FBS containing) tissue culture supernatant of CHO transfectants. Purity was >90% by SDS-PAGE. Purified fusion protein was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated fusion protein was removed using size exclusion chromatography. CD137-huIg/R-PE conjugate is at 0.2 mg/ml with an A565/A280 ratio of 3.84.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for CD137 protein
Information: Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4). The interaction of CD137 (4-1BB) and CD137L can supply a costimulatory signal to T cells through CD137. This costimulus seems to be more effective for CD8+ effector T cells than for CD4+ helper T cells (6). The interaction can also induce monocyte activation through CD137L (5). CD137 huIg fusion binds to cell surface CD137L on Raji cells in flow cytometry.
References
1) M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227. 2) H.J. Gruss, and S.K. Dower, (1995) Blood 85: 3378-3404. 3) B.A. Garni-Wagner, et al, (1996) Cellular Immunol 169: 91-98. 4) H. Schwarz, et al, (1996) Blood 87: 2839-2845. 5) J. Langstein, et al, (1998) J Immunol 160: 2488-2494. 6) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
4,045 Da
NCBI Official Full Name
CD137, partial
UniProt Protein Name
CD137
UniProt Gene Name
TNFRSF9
UniProt Entry Name
A9YYF3_MACFA
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Product Notes
The CD137 tnfrsf9 (Catalog #AAA666800) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The CD137(4-1BB)-huIg-PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD137 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). 1:20 (or use 4 ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD137 tnfrsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD137, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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