Mouse anti-Human CD137L Monoclonal Antibody | anti-CD137L antibody

anti-human CD137L-Biotin

Cat. Num. AAA666016

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Synonyms: CD137L; Monoclonal Antibody; anti-human CD137L-Biotin; Monoclonal anti-human CD137L (4-1BB Ligand)/Biotin; anti-CD137L antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2ak
• Clone Number: ANC5D6
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD137L antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Performance: Five x 105 cultured Raji cells were incubated 45 minutes on ice with 80 ul of anti-CD137L/Biotin at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD137L antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant human CD137L
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD137L antibody

Information: Human CD137 Ligand is a type II transmembrane protein constitutively expressed by monocytes, B cells neuroblastoma cells (1), and some activated T cells. The interaction of CD137 (4-1BB) and CD137L can supply a costimulatory signal to T cells through CD137. This costimulus seems to be more effective for CD8+ effector T cells than for CD4+ helper T cells (3). The interaction can also effect a reverse signal, inducing monocyte activation (2), or T cell inactivation and programmed death (4) through CD137L. Anti-CD137L antibody clone ANC5D6 binds to CD137L present on Raji and U-937 cells, and also binds to recombinant CD137L-muCD8 in EIA. This antibody does not block the interaction between CD137 and CD137L.

References

1) M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227. 2) J. Langstein, et al, (1998) J Immunol 160: 2488-2494. 3) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 4) H. Schwartz, et al, (1996) Blood 87: 2839. 5) G. Zhu, et al, (2001) J Immunol 167: 2671-6.