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Western Blot (WB) (Caspase-3 mAb tested by Western blot. Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 uM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively.)

Mouse Caspase-3 Monoclonal Antibody | anti-CASP3 antibody

Caspase-3 antibody (mAb)(Clone 31A1067)

Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Caspase-3; Monoclonal Antibody; Caspase-3 antibody (mAb)(Clone 31A1067); CASP3; anti-CASP3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
31A1067
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-CASP3 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 2-4ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This Caspase-3 antibody was raised against a 6xHis-Tag full-length human Caspase-3 protein.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(Caspase-3 mAb tested by Western blot. Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 uM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively.)

Western Blot (WB) (Caspase-3 mAb tested by Western blot. Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 uM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively.)
Related Product Information for anti-CASP3 antibody
Short Description: Our Caspase-3 antibody (mAb)(Clone 31A1067) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human, Mouse and Rat samples.

Background: Caspase-3 (CASP3 or apoptosis-related cysteine peptidase 3) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-3 cleaves and activates Caspase-6, Caspase-7 and Caspase-9, and the protein itself is processed by Caspase-8, Caspase-9 and Caspase-10. Caspase-3 is the predominate caspase involved in the cleavage of amyloid-beta 4A, which is associated with Alzheimer's disease.
Product Categories/Family for anti-CASP3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
836
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30 kDa
NCBI Official Full Name
caspase-3 preproprotein
NCBI Official Synonym Full Names
caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP3
NCBI Official Synonym Symbols
CPP32; SCA-1; CPP32B
NCBI Protein Information
caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
UniProt Protein Name
Caspase-3
Protein Family
UniProt Gene Name
CASP3
UniProt Synonym Gene Names
CPP32; CASP-3; CPP-32; SCA-1
UniProt Entry Name
CASP3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Protease; EC 3.4.22.56; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; proteolysis; regulation of caspase activity; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; response to UV; cell structure disassembly during apoptosis; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; caspase activation via cytochrome c; platelet formation; response to DNA damage stimulus; induction of apoptosis by oxidative stress; negative regulation of apoptosis

Research Articles on CASP3

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Product Notes

The CASP3 casp3 (Catalog #AAA388682) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Caspase-3 antibody (mAb)(Clone 31A1067) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 2-4ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the CASP3 casp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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