Rabbit Caspase-14 Polyclonal Antibody | anti-Casp14 antibody

Caspase-14 Antibody

Cat. Num. AAA151361

• Gene Names: Casp14; MICE; mini-ICE
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caspase-14 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Caspase-14; Polyclonal Antibody; Caspase-14 Antibody; MICE; mini-ICE; Mini-ICE; CASP-14; caspase 14; anti-Casp14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caspase-14 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 257

• Applicable Applications for anti-Casp14 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Caspase-14 antibody can be used for the detection of caspase-14 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Caspase-14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human caspase-14.
• Buffer: Caspase-14 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Caspase-14 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of caspase-14 in human liver tissue with caspase-14 antibody at 2.5 μg/mL.)

Related Product Information for anti-Casp14 antibody

Caspase-14 Antibody: Caspases are a family of cysteine proteases that can be divided into apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Caspase-14 is highly expressed in embryonic but not adult tissues. It is processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in the keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

NCBI and Uniprot Product Information

• NCBI GI #: 6753280
• NCBI GeneID: 12365
• NCBI Accession #: NP_033939
• NCBI GenBank Nucleotide #: NM_009809.5
• UniProt Accession #: O89094
• Molecular Weight: 18,224 Da
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14
• NCBI Official Symbol: Casp14
• NCBI Official Synonym Symbols: MICE; mini-ICE
• NCBI Protein Information: caspase-14; CASP-14
• UniProt Protein Name: Caspase-14
• Protein Family: Caspase
• UniProt Gene Name: Casp14
• UniProt Synonym Gene Names: CASP-14; MICE
• UniProt Entry Name: CASPE_MOUSE

Uniprot Description

CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.-; Protease

Cellular Component: keratin filament; cytoplasm; nucleus

Molecular Function: peptidase activity; hydrolase activity; cysteine-type peptidase activity

Biological Process: caspase activation; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; cell differentiation; proteolysis