Western Blot (WB)
(TRAF2 mAb tested by Western blot. TRAF2 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear extract and TRAF2 mAb at a 2 ug/ml dilution.)
Mouse anti-Human TRAF2 Monoclonal Antibody | anti-TRAF2 antibody
TRAF2 antibody (mAb)(Clone 33A1293)
Gene Names
TRAF2; TRAP; TRAP3; MGC:45012
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
TRAF2; Monoclonal Antibody; TRAF2 antibody (mAb)(Clone 33A1293); TRAP; TRAP3; MGC:45012; anti-TRAF2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
33A1293
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-TRAF2 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This TRAF2 antibody was raised against a fusion protein corresponding to amino acid residues 215 to 222 of human TRAF2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(TRAF2 mAb tested by Western blot. TRAF2 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear extract and TRAF2 mAb at a 2 ug/ml dilution.)
Western Blot (WB)
(TRAF2 mAb tested by Western blot. TRAF2 detection by Western blot. The analysis was performed using 30 ug HeLa nuclear extract and TRAF2 mAb at a 2 ug/ml dilution.)
Related Product Information for anti-TRAF2 antibody
Short Description: Our TRAF2 antibody (mAb)(Clone 33A1293) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: TRAF2 (TNF receptor-associated factor 2, TRAP) directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NFkB (NFkB p50 & NFkB p65). The protein complex formed by TRAF2 and TRAF1 interact with the inhibitor-of-apoptosis proteins (Livin/ML-IAP for example), and function as a mediator of the anti-apoptotic signals from TNF receptors. TRAF2 interaction with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation (Caspase-3, Caspase-7, Caspase-8, Caspase-9 and Caspase-14). BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis.
Background: TRAF2 (TNF receptor-associated factor 2, TRAP) directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NFkB (NFkB p50 & NFkB p65). The protein complex formed by TRAF2 and TRAF1 interact with the inhibitor-of-apoptosis proteins (Livin/ML-IAP for example), and function as a mediator of the anti-apoptotic signals from TNF receptors. TRAF2 interaction with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation (Caspase-3, Caspase-7, Caspase-8, Caspase-9 and Caspase-14). BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis.
Product Categories/Family for anti-TRAF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,055 Da
NCBI Official Full Name
TNF receptor-associated factor 2
NCBI Official Synonym Full Names
TNF receptor-associated factor 2
NCBI Official Symbol
TRAF2
NCBI Official Synonym Symbols
TRAP; TRAP3; MGC:45012
NCBI Protein Information
TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; tumor necrosis factor type 2 receptor associated protein 3; tumor necrosis factor type 2 receptor-associated protein 3
UniProt Protein Name
TNF receptor-associated factor 2
Protein Family
UniProt Gene Name
TRAF2
UniProt Synonym Gene Names
TRAP3
UniProt Entry Name
TRAF2_HUMAN
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Product Notes
The TRAF2 traf2 (Catalog #AAA388679) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRAF2 antibody (mAb)(Clone 33A1293) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRAF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the TRAF2 traf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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