Western Blot (WB)
(Caspase-9 pAb (Pro-form) tested by Western blot. Caspase-9 detection by Western blot. The analysis was performed using 50 ug HeLa whole-cell extract and Caspase-9 pAb (Pro-form) at a 1:1,000 dilution.)
Mouse anti-Human Caspase-9 Polyclonal Antibody | anti-CASP9 antibody
Caspase-9 antibody (pAb)(Pro-form)
WB: 1:1,000 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Western Blot (WB)
(Caspase-9 pAb (Pro-form) tested by Western blot. Caspase-9 detection by Western blot. The analysis was performed using 50 ug HeLa whole-cell extract and Caspase-9 pAb (Pro-form) at a 1:1,000 dilution.)
Western Blot (WB)
(Caspase-9 pAb (Pro-form) tested by Western blot. Caspase-9 detection by Western blot. The analysis was performed using 50 ug HeLa whole-cell extract and Caspase-9 pAb (Pro-form) at a 1:1,000 dilution.)
Background: Caspase-9 (CASP9 or apoptosis-related cysteine peptidase 9)(Pro-form) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-9 is processed by caspase APAF1, which is thought to be one of the earliest events in the caspase activation cascade. Caspase-9 is then involved in processing of Caspase-3.
NCBI and Uniprot Product Information
Uniprot Description
CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.
Protein type: Apoptosis; EC 3.4.22.62; Protease
Chromosomal Location of Human Ortholog: 1p36.21
Cellular Component: mitochondrion; nucleus; cytosol; apoptosome
Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; protein kinase binding; SH3 domain binding
Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging
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Product Notes
The CASP9 casp9 (Catalog #AAA388687) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Caspase-9 antibody (pAb)(Pro-form) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:1,000 dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the CASP9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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