Western Blot (WB)
(IRAK-2 pAb tested by Western blot. Detection of IRAK-2 by Western blot analysis. The analysis was performed using 10 ug/lane of HeLa (lane 1) and K-562 (lane 2) nuclear extracts and IRAK-2 pAb at a 2 ug/ml dilution.)
Rabbit anti-Human IRAK-2 Polyclonal Antibody | anti-IRAK-2 antibody
IRAK-2 antibody (pAb)
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Western Blot (WB)
(IRAK-2 pAb tested by Western blot. Detection of IRAK-2 by Western blot analysis. The analysis was performed using 10 ug/lane of HeLa (lane 1) and K-562 (lane 2) nuclear extracts and IRAK-2 pAb at a 2 ug/ml dilution.)
Western Blot (WB)
(IRAK-2 pAb tested by Western blot. Detection of IRAK-2 by Western blot analysis. The analysis was performed using 10 ug/lane of HeLa (lane 1) and K-562 (lane 2) nuclear extracts and IRAK-2 pAb at a 2 ug/ml dilution.)
Background: IRAK-2-Interleukin-1 Receptor-Associated Kinase2 is one of two serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation to upregulate NFkB (NFkB p50 & NFkB p65) expression.
NCBI and Uniprot Product Information
Uniprot Description
IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; IRAK family
Chromosomal Location of Human Ortholog: 3p25.3
Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; ATP binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; inhibition of NF-kappaB transcription factor; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; innate immune response; regulation of cytokine and chemokine mediated signaling pathway; JNK cascade; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway
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Product Notes
The IRAK-2 irak2 (Catalog #AAA388675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRAK-2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IRAK-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the IRAK-2 irak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRAK-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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