Typical Testing Data/Standard Curve (for reference only)
Mouse Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit | GLP1R elisa kit
Mouse Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit
Reactivity
Mouse
Synonyms
Glucagon Like Peptide 1 Receptor (GLP1R); Mouse Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit; GLP1R elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of GLP1R. No significant cross-reactivity or interference between GLP1R and analogues was observed.
Sequence Length
463
Samples
Mouse tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLP1R were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLP1R were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GLP1R elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GLP1R in mouse tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GLP1R. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GLP1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLP1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLP1R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GLP1R. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GLP1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLP1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLP1R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GLP1R elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,026 Da
NCBI Official Full Name
glucagon-like peptide 1 receptor
NCBI Official Synonym Full Names
glucagon-like peptide 1 receptor
NCBI Official Symbol
GLP1R
NCBI Protein Information
glucagon-like peptide 1 receptor
UniProt Protein Name
Glucagon-like peptide 1 receptor
Protein Family
UniProt Gene Name
GLP1R
UniProt Synonym Gene Names
GLP-1 receptor; GLP-1-R; GLP-1R
UniProt Entry Name
GLP1R_HUMAN
Uniprot Description
GLP1R: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2
Chromosomal Location of Human Ortholog: 6p21
Cellular Component: plasma membrane; integral to membrane
Molecular Function: transmembrane receptor activity; glucagon receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; learning and/or memory; adenylate cyclase activation; energy reserve metabolic process; response to stress; regulation of insulin secretion; regulation of heart contraction
Research Articles on GLP1R
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Product Notes
The Mouse GLP1R glp1r (Catalog #AAA2023256) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023256 ELISA Kit recognizes Mouse GLP1R. It is sometimes possible for the material contained within the vial of "Glucagon Like Peptide 1 Receptor (GLP1R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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