Western Blot (WB)
(Western Blot analysis:|NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MBS602948 (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Mouse C/EBPb Monoclonal Antibody | anti-cebpb antibody
C/EBPb (CCAAT/Enhancer-Binding Protein beta)
Gene Names
cebpb; cb380; c/ebpb; fe11e05; wu:fe11e05
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
C/EBPb; Monoclonal Antibody; C/EBPb (CCAAT/Enhancer-Binding Protein beta); Anti -C/EBPb (CCAAT/Enhancer-Binding Protein beta); anti-cebpb antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
7G10
Specificity
Recognizes human C/EBPb. Species Crossreactivity: Mouse
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-cebpb antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:500-1:2000
Dilution: Western Blot: 1:500-1:2000
Immunogen
Recombinant corresponding to aa1-271 from human C/EBPb purified from E. coli (NP_005185).
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis:|NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MBS602948 (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Western Blot analysis:|NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MBS602948 (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-cebpb antibody
CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPa, C/EBPb, C/EBPd, C/EBPe, and C/EBPg. Among these proteins, C/EBPb is usually highly expressed in liver, intestine, lung and adipose. C/EBPb has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.
Product Categories/Family for anti-cebpb antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,029 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein beta
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), beta
NCBI Official Symbol
cebpb
NCBI Official Synonym Symbols
cb380; c/ebpb; fe11e05; wu:fe11e05
NCBI Protein Information
CCAAT/enhancer-binding protein beta
UniProt Protein Name
CCAAT/enhancer binding protein (C/EBP), beta
Protein Family
UniProt Gene Name
cebpb
UniProt Entry Name
Q7ZU07_DANRE
Uniprot Description
Sequence similarities: Belongs to the bZIP family. RuleBase RU000470Contains 1 bZIP domain. SAAS SAAS004827
Similar Products
Product Notes
The cebpb cebpb (Catalog #AAA602948) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The C/EBPb (CCAAT/Enhancer-Binding Protein beta) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBPb can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:500-1:2000. Researchers should empirically determine the suitability of the cebpb cebpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBPb, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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