Western Blot (WB)
(Western Blot analysis: Rat serum (15ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with MBS601670 (1:2000). Proteins were visualized using an IgG (HRP) goat anti-mouse and an ECL detection system.)
Mouse Vaspin Monoclonal Antibody
Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12)
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Vaspin; Monoclonal Antibody; Vaspin (Visceral Adipose-Specific SERPIN; SERPINA12; Serine Proteinase Inhibitor-clade A12); Anti -Vaspin (Visceral Adipose-Specific SERPIN; anti-Vaspin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
8G9
Specificity
Recognizes human Vaspin at ~47kD. Species Crossreactivity: rat
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-Vaspin antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: Western Blot (cell lysate): 1:1000-1:3000
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Immunogen
Recombinant corresponding to aa21-414 of human Vaspin, expressed in E. coli .
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis: Rat serum (15ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with MBS601670 (1:2000). Proteins were visualized using an IgG (HRP) goat anti-mouse and an ECL detection system.)
Western Blot (WB)
(Western Blot analysis: Rat serum (15ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with MBS601670 (1:2000). Proteins were visualized using an IgG (HRP) goat anti-mouse and an ECL detection system.)
Related Product Information for anti-Vaspin antibody
Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism.
Product Categories/Family for anti-Vaspin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
visceral adipose tissue specific SERPIN
Uniprot Description
SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family.
Protein type: Secreted, signal peptide; Secreted; Inhibitor
Cellular Component: extracellular space; plasma membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; glucose metabolic process; positive regulation of insulin receptor signaling pathway
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Product Notes
The Vaspin (Catalog #AAA601670) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Vaspin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: Western Blot (cell lysate): 1:1000-1:3000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Vaspin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vaspin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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